An in-vitro method for studying reactivity of intracerebral venules

研究脑内小静脉反应性的体外方法

• 批准号: 03807091
• 负责人: TAKAYASU Masakazu
• 金额: $ 1.22万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807091/
• 关键词: Cerebral microcirculation; Venules; Vascular smooth muscle; Norepinephrine; Arterioles; 静脈

While cerebral arterioles control cerebrovascular resistance,cerebral venules are responsible for considerable fraction of cerebral blood volume. However, no in-vitro method was available to investigate reactivity of cerebral venules. The purpose of this study was (1) to develop an in-vitro method for studying reactivity of intracerebral venules; (2) to determine the noradrenergic reactivity of intracerebral venules using this method. The method was modification of that for intracerebral arterioles. Intracerebral venules with a length of about 800 mum and diameter in the range of 4090 mum were isolated from rat brains. The vessel segments were transferred to a temperature-controlled chamber on the stage of an inverted microscope and were cannulated with specially designed glass pipettes. Transmural pressure of venules was set at 5 mmHg and the organ-bath temperature was brought to 37.5ﾟC. The venules well responded to pH change of the organ-bath. Extraluminally applied norepinephrine (10^<-9>-10^<-3>M) constricted the venules in a dose-dependent manner, reaching 63.7*0.9 of the control diameter at a dose of 10^<-4>M in contrast to intracerebral arterioles which had no response to norepinephrine. The present results indicate an important heterogeneity of noradrenargic responsiveness between intracerebral arterioles and venules, suggesting an importance of studying cerebral venules to understand the cerebral circulation.

脑小动脉控制脑血管阻力，而脑小静脉则负责相当大部分的脑血容量。然而，没有体外方法可用于研究脑小静脉的反应性。本研究的目的是：（1）建立一种体外研究脑内微静脉反应性的方法;（2）用该方法测定脑内微静脉的去甲肾上腺素能反应性。该方法是对脑内小动脉方法的改进。从大鼠脑中分离出长度为约800 μ m且直径在4090 μ m范围内的脑内小静脉。将血管节段转移到倒置显微镜载物台上的温控室中，并用专门设计的玻璃移液器插管。将小静脉的压力设定为5 mmHg，并将器官浴温度升至37.5 ℃。微静脉对器官浴的pH值变化反应良好。腔外注射去甲肾上腺素（10 <-9>- 10 μ <-3>M）以剂量依赖性方式收缩小静脉，在10 μ M剂量下达到对照直径的63.7*0.9<-4>，而脑内小动脉对去甲肾上腺素无反应。目前的结果表明，一个重要的异质性去甲肾上腺素能反应脑内小动脉和小静脉之间，这表明研究脑微静脉了解脑循环的重要性。

项目成果

小倉 浩一郎,高安 正和 梶田 泰一,他: "Differential effecto on norepinephrine on intracerebral arterioles and venules of rats in vitro" Microcirculation annuals. 18. 37-38 (1992)

Koichiro Ogura、Masakazu Takayasu、Yasuichi Kajita 等人：“去甲肾上腺素对体外大鼠脑内小动脉和小静脉的差异作用”微循环年鉴 18. 37-38 (1992)。

小倉 浩一郎,高安 正和,梶田 泰一,他: "Differential effects on norepinephrie on intracerebral arterioles and venules of rats in vitro" Microcirculation annuals. 18. 37-38 (1992)

Koichiro Ogura、Masakazu Takayasu、Yasuichi Kajita 等人：“体外大鼠脑内小动脉和小静脉对去甲肾上腺素的不同影响”微循环年鉴 18. 37-38 (1992)。

Koichiro Ogawa , Masakazu Takayasu , Yasuichi Kajita et al,: "Differential effects on norepinephrine on intracerebral arterioles and venules of vats in vitro" Microcirculation annuals. 18. 37-38 (1992)

Koichiro Okawa、Masakazu Takayasu、Yasuichi Kajita 等人：“去甲肾上腺素对体外脑内小动脉和小静脉的不同影响”一年生微循环。