Basic study for the development of small caliber vascular prosthesis with alternately aligned static magnetic field

我们初步认为交替排列的静磁场可促进血管内皮再生。在这项研究中，我们从磁场的强度和方向的角度来评估效果。通过将温和的干燥空气流通入动脉，使犬颈动脉和股动脉内皮剥脱。每对裸露的动脉中的一条被磁化材料包裹，另一条被非磁化材料包裹。间隔一段时间后，我们收集它们进行电子显微镜检查和测量动脉壁中的DNA浓度。[实验1]我们使用了由长磁极和薄磁极组成的两种类型的磁片。磁星通量密度为280和360高斯。我们采用磁场方向平行于动脉长轴和垂直于动脉长轴两种方式施加磁片。结果磁处理组内皮细胞再生速度快于对照组。我们发现磁场强度没有显着差异。垂直贴敷有上级优于平行贴敷的趋势。[实验2]我们使用三种类型的环形磁体，内径为6 mm，宽度为3.5 mm。类型1磁体纵向磁化，类型2横向磁化，类型3周向磁化，导致10个磁极围绕圆周。我们使用五个环形磁铁通过磁力耦合，形成一个圆柱形磁铁。结果磁处理组血管内皮再生面积明显大于对照组。1型磁体显示更好的内皮再生。磁场组DNA含量低于对照组。这可能表明基于暴露的内皮下被再生的内皮细胞较早覆盖的平滑肌细胞增殖的抑制。

We suggested alternately aligned static magnetikc fields could promote endothelial regeneration in a preliminary stucy. In this study, we evaluated the effect from the viewpoint of the intensity and direction of magnetic field. Canine carotid and femoral arteries were denudd of endothelium by passing a gentle stream of dry air into the arteries. One of each pair of denuded arteries was wrapped in a magnetized materials and the other was in a non-magnetized one. After a certain period of interval, we collected them for electron microscopic examination and measurement of DNA concentration in the arterial wall. [Experiment 1] We used two types of magnetic sheet consisting of long and thin magnetic poles. The magnetid flux densities were 280 and 360 Gauss. We applied the magnetic sheet in two ways that its direction of magnetic field was parallel or perpendicular to the long axis of the arteries. Results The magnetic groups showed fadster endothelial regeneration than the control group. We found the magnetic intensities made no significant difference. There was a tendency that the perpendicular application was superior to the parallel one. [Experiment 2] We used three types of ringed magnet, an internal diameter of 6 mm and a width of 3.5 mm. Type 1 magnet was magnetizd longitudinally, Type 2 was transversely and Type 3 was circumferentially, resulting in 10 poles round the circumfarence. We used five ringed magnets coupled by magnetic force, forming a cylindrical magnet. Results The area of endothelial regeneration was significantly larger in the magnetic groupsthan in the control group. Type 1 magnet showed better endothelial regeneration. The DNA concentration was lower int he magnetic groups than in the control group. This may suggest the suppression of smooth muscle cell proliferation based on the earlier covering of exposed subendothelium with regenerated endothelial cells.