Detection of phytopathogenic Xanthomonads via a novel perception mechanism in Arabidopsis thaliana

通过拟南芥中的新型感知机制检测植物病原性黄单胞菌

• 批准号: 441178209
• 负责人: Professor Dr. Georg Felix
• 金额: --
• 依托单位: Abteilung für Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/441178209?language=en
• 关键词: Detection; phytopathogenic; Xanthomonads; via; novel

Many bacterial species of the genus Xanthomonas are serious pathogens for crop plants. In our previous work we detected that, as part of their defense system, the model plant Arabidopsis has means to detect Xanthomonas via the pattern recognition receptor ReMAX/RLP1 that detects a specific, but yet unidentified, protein from Xanthomonas bacteria. In our ongoing attempts to purify and identify this protein, we detected a second factor that is perceived by Arabidopsis in a ReMAX/RLP1 independent way. Purification of this second factor proved easier and allowed its identification as a subtilase enzyme secreted by the Xanthomonas bacteria. Our results, so far, show that it is not the subtilase molecule itself, which is sensed. Rather, our evidence shows that the proteolytic activity of the bacterial subtilase processes a protein exported by the plant cells to a smaller polypeptide that triggers typical defense responses in plant cells via a specific surface receptor. In order to characterize this novel molecular pathogen perception mechanism, the primary aims of this project are the identification of this plant factor, termed SAMP for subtilase activated molecular pattern, and its cognate plant receptor.

黄单胞菌属的许多细菌种类是农作物的严重病原菌。在我们之前的工作中，我们检测到，作为其防御系统的一部分，模式植物拟南芥具有通过模式识别受体ReMax/RLP1检测黄单胞菌的手段，该受体检测来自黄单胞菌的特定但尚未鉴定的蛋白质。在我们正在进行的纯化和鉴定该蛋白的尝试中，我们检测到了第二个因子，该因子被拟南芥以ReMax/RLP1独立的方式感知。第二个因子的纯化被证明更容易，并使其能够被鉴定为Xanthomonas细菌分泌的一种枯草杆菌酶。到目前为止，我们的结果表明，它不是被感知的枯草杆菌分子本身。相反，我们的证据表明，细菌枯草杆菌的蛋白分解活性将植物细胞输出的蛋白质加工成更小的多肽，通过特定的表面受体在植物细胞中触发典型的防御反应。为了研究这种新的病原菌分子感知机制，本项目的主要目的是鉴定这种被称为枯草杆菌激活分子模式的植物因子和它的同源植物受体。