Detection of phytopathogenic Xanthomonads via a novel perception mechanism in Arabidopsis thaliana

通过拟南芥中的新型感知机制检测植物病原性黄单胞菌

• 批准号: 441178209
• 负责人: Professor Dr. Georg Felix
• 金额: --
• 依托单位: Abteilung für Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/441178209?language=en
• 关键词: Detection; phytopathogenic; Xanthomonads; via; novel

Many bacterial species of the genus Xanthomonas are serious pathogens for crop plants. In our previous work we detected that, as part of their defense system, the model plant Arabidopsis has means to detect Xanthomonas via the pattern recognition receptor ReMAX/RLP1 that detects a specific, but yet unidentified, protein from Xanthomonas bacteria. In our ongoing attempts to purify and identify this protein, we detected a second factor that is perceived by Arabidopsis in a ReMAX/RLP1 independent way. Purification of this second factor proved easier and allowed its identification as a subtilase enzyme secreted by the Xanthomonas bacteria. Our results, so far, show that it is not the subtilase molecule itself, which is sensed. Rather, our evidence shows that the proteolytic activity of the bacterial subtilase processes a protein exported by the plant cells to a smaller polypeptide that triggers typical defense responses in plant cells via a specific surface receptor. In order to characterize this novel molecular pathogen perception mechanism, the primary aims of this project are the identification of this plant factor, termed SAMP for subtilase activated molecular pattern, and its cognate plant receptor.

Xanthomonas属的许多细菌种类是农作物植物的严重病原体。在我们先前的工作中，我们发现，作为其防御系统的一部分，模型植物拟南芥具有通过模式识别受体Remax/rlp1检测Xanthomonas，该模型可检测到Xanthomonas细菌的特定但未识别的蛋白质。在我们正在进行净化和识别该蛋白质的持续尝试中，我们检测到了拟南芥在remax/rlp1独立的方式中所感知的第二个因素。事实证明，第二个因子的纯化使其鉴定为叶雀细菌分泌的牙酶。到目前为止，我们的结果表明，感知到的不是毕利酶分子本身。相反，我们的证据表明，细菌性毕利酶的蛋白水解活性处理由植物细胞导出到较小多肽的蛋白质，该蛋白通过特定的表面受体在植物细胞中触发典型的防御反应。为了表征这种新颖的分子病原体感知机制，该项目的主要目的是鉴定该植物因子，称为锻炼酶激活的分子模式及其同源植物受体。