Control Mechanism of Metabolism of Oligosaccharins Regulating Plant Growth

低糖精代谢调节植物生长的控制机制

• 批准号: 04640628
• 负责人: HOSON Takayuki
• 金额: $ 1.02万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640628/
• 关键词: Oligosaccharin; Cell wall; Xyloglucan; Azuki bean; Growth regulation; Immunoassay; Apoplast; Hydrolase; ピリジルアミノ化; フコシル・ラクトース

Certain types of oligosaccharide derived from plant or fungal cell walls possess diverse physiological activities. These active oligosaccharides, called "oligosaccharins" appear to be involved in the regulation of life cycle of higher plants. It has been reported that xyloglucan oligosaccharides play an important role in growth regulation. The present study was undertaken to clarify how the metabolism of the xyloglucan oligosaccharins is regulated in situ in the cell wall network.1.The effect of xyloglucan nonasaccharide (XG9 or XXFG) and fucosyl-lactose on different growth processes was examined. Neither XG9 nor fucosyl-lactose did influence auxin- or acid-induced elongation of azuki bean epicotyl segments, gibberellic acid-induced growth of intact lettuce hypocotyls, or benzyladenine-induced expansion of radish cotyledons.2.The endogenous concentration of xyloglucan oligosaccharides was determined with ELISA assay employing antibodies specific for the oligosaccharides and with new microassay using the antibodies and pyridylamination fluorescence labeling. The level of xyloglucan oligosaccharides was estimated as 10^<-8> M or lower.3.Two types of enzyme that appear to be involved in the production and the degradation of xyloglucan oligosaccharides were extracted with 1 M NaCl from the cell wall preparation of azuki bean epicotyls. Xyloglucan-hydrolyzing enzymes (endo-1,4-beta-glucanase) and alpha-fucosidases were purified with Con A-Sepharose, Mono-S, Superdex 75, and Phenyl Superose columms. Both enzymes were composed of several isozymes. The endo-1,4-beta-glucanases were clearly separated from cellulase on a Mono-S column and exhibited different action patterns. alpha-Fucosidases did not hydrolyze rho-nitrophenyl-alpha-L-fucopyranoside and were specific for xyloglucan side chains.

某些来源于植物或真菌细胞壁的寡糖具有多种生理活性。这些活性低聚糖被称为“寡糖素”，参与高等植物生命周期的调控。据报道，木葡聚糖寡糖在生长调节中起重要作用。本研究旨在阐明木葡聚糖寡糖素在细胞壁网络中的代谢是如何原位调控的。1.研究了木葡聚糖九聚糖（XG 9或XXFG）和岩藻糖基乳糖对不同生长过程的影响。XG 9和岩藻糖基乳糖均不影响生长素或酸诱导的小豆上胚轴切段伸长、赤霉酸诱导的完整莴苣下胚轴生长和苄基腺嘌呤诱导的萝卜子叶膨大。2.采用特异性木葡聚糖抗体的ELISA法和新的微量测定法测定了内源木葡聚糖的浓度。木葡聚糖低聚糖的水平估计为10 μ <-8>M或更低。3.用1 M NaCl从红豆上胚轴细胞壁制备物中提取了两种似乎参与木葡聚糖低聚糖产生和降解的酶。木葡聚糖水解酶（内切-1，4-β-葡聚糖酶）和α-岩藻糖苷酶用Con A-Sepharose、Mono-S、Superdex 75和Phenyl Superose柱纯化。这两种酶都由几种同工酶组成。内切-1，4-β-葡聚糖酶与纤维素酶在Mono-S柱上分离清楚，并表现出不同的作用模式。α-岩藻糖苷酶不水解对硝基苯基-α-L-吡喃岩藻糖苷，并且对木葡聚糖侧链具有特异性。

项目成果

Hoson,T.: "Regulation of polysaccharide breakdown during auxin-induced cell wall loosening." J.Plant Res.106. 369-381 (1993)

Hoson,T.：“生长素诱导的细胞壁松弛过程中多糖分解的调节。”

Masuda,Y.(編): "Plant Cell Walls as Biopolymers with Physiological Functions" Yamada Science Foundation, 452 (1992)

Masuda, Y.（编）：“植物细胞壁作为具有生理功能的生物聚合物”山田科学基金会，452（1992）

Hoson,T.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl segments." Physiologia Plantarum. 87. 142-147 (1993)

Hoson,T.：“表皮细胞壁中木葡聚糖分解对于生长素诱导的小豆上胚轴节段伸长的作用。”

Hoson, .T.et al.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl segments." Physiol. Plant.87. 142-147 (1993)

Hoson，T.等人：“表皮细胞壁中木葡聚糖分解对于生长素诱导的小豆上胚轴节段伸长的作用。”

Hoson,T.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl segments." Physiol.Plant.87. 142-147 (1993)

Hoson,T.：“表皮细胞壁中木葡聚糖分解对于生长素诱导的小豆上胚轴节段伸长的作用。”