The role of sulfate-reducing bacteria on insolubilization of heavy metal ions at waste disposal sites

硫酸盐还原菌对垃圾处理场重金属离子不溶化的作用

• 批准号: 04650495
• 负责人: TAKAMIZAWA Kazuhiro
• 金额: $ 1.41万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04650495/
• 关键词: sulfate-reducing bacteria; waste disposal site; methane-producing bacteria; 不溶化; 金属硫化物

Distribution of sulfate-reducing bacteria (SRB) at two sea-based solid waste disposal sites and seven landfill sites were conducted from 1993 to 1995, resulting that SRB widely distributed in the sites. SRBs were accumulated and insolubilization experiments using several heavy metal ions were performed. Insolubilization rate of hexavalent chromium was very low of 16%, neverthless, Cd, Cu, Fe, and Zn ions were precipitated within 48 hr reaction with hydrogen sulfide produced by SRBs. In case of Cd ion, produced precipitate was determined as cadmium sulfide from SEM and EDX analysis. The role of SRBs for insolubilization of heavy metal ions in waste disposal sites turned evident. Among SRBs, Desulfotomaculum sp. was isolated. Methane-producing bacteria (MPB) were also widely distributed in sites and MPB number counted from boring-pits samples were larger than those of from leachates. If samples contained 20mg/l or more of sulfuric ion concentration, SRBs number were greater than MPB number. Two kinds of MPB were isolated. One was Methanosarcina sp., which utilized both methanol and acetic acid and the other was thermophilic Methanomicrobium sp. The difference between MPB and other bacteria was confirmed by fluorescence microscopy using 420nm of exciting wavelength, but it was difficult to differ MPB from SRBs by fluorescence microscopy.

1993 ~ 1995年对2个海洋固体废物处置场和7个垃圾填埋场的硫酸盐还原菌（SRB）分布进行了研究，结果表明SRB在各处置场均有广泛分布。SRB的积累和使用几种重金属离子的不溶实验进行。六价铬的不溶率很低，只有16%，几乎没有镉、铜、铁和锌离子在48小时内与SRB产生的硫化氢反应沉淀。在Cd离子的情况下，从SEM和EDX分析确定所产生的沉淀物为硫化镉。硫酸盐还原菌对垃圾场重金属离子的不溶作用越来越明显。硫酸盐还原菌中分离到脱硫肠状菌（Desulfotomaculum sp.）。产甲烷菌（MPB）也广泛分布于现场，从钻孔样品中计数的MPB数量大于从渗滤液中计数的数量。当样品中硫离子浓度大于20 mg/l时，SRB数大于MPB数。分离到两种MPB。一种是甲烷八叠球菌，利用荧光显微镜在420 nm激发波长下观察到MPB与其它细菌的差异，但在荧光显微镜下很难将MPB与SRB区分开来。

项目成果

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bocteria at a sea-bared solid waste disposal site and their role on insolubilization of heary metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原细菌在裸露的固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理协会杂志 5. 89-97 (1994)。

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bacteria at a sea-based solid waste site and their role on insolubilization of heavy metal ions in leachate." J.Japan Soc.Waste Management Experts.5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物场的分布及其对渗滤液中重金属离子不溶性的作用。”

Kazuhiro Takamizawa: "Distribution of sulfute-reducing bacteria at a sea-based solid waste disposal site and their role on insolubilization of heavy metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理学会杂志 5. 89-97 (1994)。