Isolation and taxonomic study of Gramnegative, aerobic, marine bacteria

对99株革兰氏阴性、需氧、氮周鞭毛海洋细菌进行了分类研究。其中81种在本研究中从日本沿海地区分离出来。根据化学分类特征将菌株分为四类。第I组对应于Deleya属和四个新种Deleya japonica sp.nov。， Deleya agilis sp. 11。， Deleya tenuis sp. 11。提出了Deleya turbida sp. 11。II组菌株在化学分类上与以前从未被认为是周生的Alteromonas属相似。第二组菌株的鞭毛经电镜分析为混合鞭毛，表型数据和DNA同源性实验表明它们属于Altermonas属。在7株混合鞭毛菌株中，鉴定出4株为大西洋交替单胞菌（Alteromonas atlantica sp.nov）新种，1株为温交替单胞菌（Alteromonas undina）。另外2株为新种，分别为litoralis Alteromonas sp.nov和简短Alteromonas sp.nov。其中，亚热带小圆草属2新属3新种。， z . nisishinoshimaensis and Oceanobacterium insulare gen. 11。, sp.nov。，得到了澄清。含有泛素-11的罕见菌株PM-4在不动杆菌属的邻近谱系中确定了系统发育位置。系统发育距离和醌类成分的差异表明，菌株PM-4应归入一个新属。费氏盐单胞菌属；11月11日被提议。

99 strains of Gram-negative, aerobic, peritrichously flagellated marine bacteria were taxonomically investigated. Among them 81 were isolated in this study from coastal reagions in Japan. Strains were divided into four categories based on chemotaxonomic features. Group I corresponds to the genus Deleya and four new species, Deleya japonica sp.nov., Deleya agilis sp.nov., Deleya tenuis sp.nov., and Deleya turbida sp.nov.were proposed. Group II strains were chemotaxonomically similar to the genus Alteromonas which never reffered as peritrichous previously. The flagellation of Group II strains were determined as mixed-flagellation by electronmicroscopy and phenotypic data and DNA homology experiment showed that they are the members of teh genus Altermonas. Among seven strains having mixed-flagellation, four were identified as a new species Alteromonas atlantica sp.nov.and one was identified as Alteromonas undina. Other two strains were classified as new species, Alteromonas litoralis sp.nov.and Alteromonas brevis sp.nov.Group III strains were taxonomically diverse as shown by rRNA-DNA hybridization. Among them, two new genera and three new species Zobellia subtropicalis gen.nov.sp.nov., Z.nishinoshimaensis and Oceanobacterium insulare gen.nov., sp.nov., were clarified. Phylogenetic position of the unusual strain PM-4 which had ubiquinone-11 was determined at the neighboring lineage to the genus Acinetobacter. The phylogenetic distance and the differnece in quinone composition indicated that strain PM-4 must be classified in a new genus. Salinomonas feraxa gen.nov., sp.nov.was proposed.