In vitro biosynthesis of enantiomeric ether polar lipids in Archaea
In vitro biosynthesis of enantiomeric ether polar lipids in Archaea
批准号:
11460051
负责人:
KOGA Yosuke
金额:
$9.22万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2001
中文摘要
最基本的特征。区别于细菌/真核生物的是膜醚型极性脂质,特别是它们的sn-甘油-1-磷酸(G-1-P)骨架。G-1-P脱氢酶是形成脂质G-1-P骨架的关键酶,据分析,它普遍存在于几种微生物中,包括产甲烷菌、超嗜热菌、嗜酸热菌和极端嗜盐菌。通过同源性搜索,在所有已发表基因组序列的大肠杆菌中也检测到编码该酶的基因(egsA)。由DHAP通过与两个香叶基香叶基的醚化形成的异麦芽酚酸又被CTP活化。该产品为CDP-古菌。本研究建立了CDP-古菌醇合酶和archetidylserine合酶的底物特异性。CDP-古醇合酶对以香叶基香叶基为烃链的底物具有特异性。它不识别甘油磷酸骨架或醚键的立体结构。AS合成酶没有歧视底物与不饱和和饱和烃,醚键和酯键,或立体结构的甘油磷酸骨架。古生菌AS合成酶的酶学性质与芽孢杆菌磷脂酰丝氨酸合成酶相似,但最适温度不同,氨基酸序列也有很大的相似性。这些结果表明古细菌AS合成酶和细菌磷脂酰丝氨酸合成酶具有共同的祖先。
英文摘要
The most fundamental characteristics. that distinguishes Archaea from Bacteria/Eucarya is membrane ether-type polar lipids, especially their sn-glycerol-1-phosphate (G-1-P) backbone. G-1-P dehydrogenase, which is the key enzyme for the formation of lipid G-1-P backbone, was found ubiquitously in several species of Archaea including methanogens, hyperthermophiles, an acidothermophile, and an extreme halophile as far as analysed. The gene (egsA) encoding the enzyme was also detected by homology search in all the Archaea whose genome sequences have been published. Archaeitidic acid, which is formed from DHAP by etherification with two geranylgeranyl groups, was in turn activated by CTP. The product is CDP-archaeol. The substrate specificities of CDP-archaeol synthase- and archetidylserine synthase were established in this study. CDP-archaeol synthase was specific to the substrate with geranylgeranyl groups as hydrocarbon chains. It did not recognized stereostructure of glycerophosphate backbone or ether bonds. AS synthase did not discriminate substrates with unsaturated and saturated hydrocarbons, ether bonds and ester bonds, or stereostructures of glycerophosphate backbone. The enzymatic properties of archaeal AS synthase were quite similar to Bacillus phosphatidylserine synthase except for the optimal temperature, and the amino acid sequences showed significant similarity. These results suggested archaeal AS synthase and bacterial phosphatidylserine synthase share their ancestor.
期刊论文(24)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Nishihara, M.: "Enzymatic determination of sn-glycerol-1-phosphate"Journal of UOEH. 22. 13-18 (2000)
Nishihara, M.:“酶法测定 sn-甘油-1-磷酸”UOEH 杂志。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Nishihara M.: "sn-glycerol-1-phosphate-forming activities in Archaea : Separation of archaeal phospholipid biosynthesis and glycerol catabolism by glycerophosphate enantiomers"Journal of Bacteriology. 181(4). 1330-1333 (1999)
Nishihara M.:“古细菌中的sn-甘油-1-磷酸形成活性:甘油磷酸对映体对古细菌磷脂生物合成和甘油分解代谢的分离”细菌学杂志。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Nishihara M.: "Gas-liquid chromatographi determination of total glycerophosphate in an aqueous sol uti on"Analytical Biochemistry. 276(2). 260-261 (1999)
Nishihara M.:“气液色谱法测定水溶液中的总甘油磷酸盐”分析生物化学。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Nishihara, M.: "Gas-liquid chromatographic determination of total glycerophosphate in an aqueous solution"Analytical Biochemistry.. 276. 260-261 (1999)
Nishihara, M.:“水溶液中总甘油磷酸盐的气液色谱测定”分析生物化学.. 276. 260-261 (1999)
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
M.Nishihara: "sn-Glycerol-1-phosphate-froming activities in Archaea"Journal of Bacteriology. 181(4). 1330-1333 (1999)
M.Nishihara:“古细菌中的sn-甘油-1-磷酸盐形成活性”细菌学杂志。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
共 12 条
Study on the evolution of Archaea and Bacteria from the view points of archaeal polar lipid biosynthesis
-
批准号:16580067
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.3万
-
财政年份:2004
-
负责人:KOGA Yosuke
-
依托单位:
New taxonomy of methanogens based on the analysis of lipid component parts
-
批准号:06660124
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1994
-
负责人:KOGA Yosuke
-
依托单位:
Isolation and taxonomic study of Gramnegative, aerobic, marine bacteria
-
批准号:04660102
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1992
-
负责人:KOGA Yosuke
-
依托单位:
The Structure and Biosynthetic Mechanism of the Monomolecular Membrane made of Tetraether Type of Polar Lipids in Methan Menic Bacteria
-
批准号:01560108
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1989
-
负责人:KOGA Yosuke
-
依托单位:
海外基金