Microbial reduction using ethanol as an energy source

使用乙醇作为能源减少微生物

• 批准号: 04660149
• 负责人: KOMETANI Tadashi
• 金额: $ 1.15万
• 依托单位: TOYAMA NATIONAL COLLEGE OF TECHNOLGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660149/
• 关键词: Microbial Reduction; Regeneration of NAD(P)H; Aldehyde Dehydrogenase; 酵母; 不斉還元反応; 培養条件

The microbial reduction of prochiral ketones has been recognaized as a useful technique to prepare a chiral secondary alcohols. Recently, a new procedure for the baker's yeast-mediated bioreduction using ethanol instead of sugar as the energy source was developed. This research is to study the microbial reduction using ethanol as the energy source and to study the relation between culitivation conditions and the reduction ability.1.Screening of microbials using ethanol as an energy source for NAD(P)H regneration coupled with a bioreductionMany of the tested strains of years use ethanol and/or acetate as an energy source for NAD(P)H regneration coupled with a bioreduction in the same manner as glucose. Then, molds use only glucose as the energy source but ethanol or acetate. On the other hand, some of bacterias use ethanol effectively but glucose.A scheme for NAD(P)H regeneration from the corresponding oxidezed from through the oxidative pathway of ethanol in yeast cells was studied. THe results suggested tha NADH was regenerated through alcoholic oxidation into acetate and NADPH through acetate oxidation into carbon cioxide.2.Relationship between the ability using ethanol as energy sourcet for NAD(P)H regeneration and the culture condition on oxygen absorption rate.Yeasts, Saccharomysex cerevisiae HUT 7099, cultured under more anaerobic conditions had the lower ablity using ethanol as energy source for NAD(P)H regeneration. The reason was related to lower activity of aldehyde dehydrogenase though the activity of alcohol dehydrogenase was not influensed by such culture conditions.

微生物还原前手性酮是一种制备手性仲醇的有效方法。最近，开发了一种以乙醇代替糖作为能源的面包酵母介导的生物还原新方法。本研究以乙醇为能源，研究了培养条件与还原能力的关系。1.以乙醇为能源，NAD（P）H再生与生物还原耦合的微生物的筛选多年来，许多被测菌株以乙醇和/或乙酸为能源，以葡萄糖为同样的方式，进行NAD（P）H再生与生物还原耦合。然后，霉菌只使用葡萄糖作为能源，而不使用乙醇或乙酸盐。另一方面，有些细菌对乙醇的利用效率很高，但对葡萄糖的利用效率不高。2.以乙醇为能源再生NAD（P）H的能力与培养条件对吸氧率的关系在厌氧条件下培养的酿酒酵母HUT 7099，以乙醇为能源再生NAD（P）H的能力较低。其原因可能与乙醛脱氢酶活性较低有关，而乙醇脱氢酶活性不受此培养条件的影响。

项目成果

Tadashi Kometani: "NAD(P)H Regeneration Using Ethanol as an Energy Source in Baker's Yeast-Mediated Bioreduction" Journal of Fermentation and Bioengineering. 77. 13-16 (1994)

Tadashi Kometani：“在贝克酵母介导的生物还原中使用乙醇作为能源进行 NAD(P)H 再生”发酵与生物工程杂志。

Tadashi Kometani: "NAD(P)HRegeneration Using Ethanol as an Energy Source in Baker′s Yeast‐Mediated Bioreduction" Journal of Fermentation and Bioengineering. 77. 13-16 (1994)

Tadashi Kometani：“在贝克酵母介导的生物还原中使用乙醇作为能源进行 NAD(P)H 再生”《发酵与生物工程杂志》77. 13-16 (1994)。

Tadashi Kometani, Yasuumi Morita, Hideyuki Furui, Hidefumi Yoshii, and Ryuichi Matsuno: "NAD(P)H Regeneration Using Ethanol as an Energy Source in Baker's Yeast-Mediated Bioreduction" Jornal of Fermentation and Bioengineering. 77. 13-16 (1994)

Tadashi Kometani、Yasuumi Morita、Hideyuki Furui、Hidefumi Yoshii 和 Ryuichi Matsuno：“在面包酵母介导的生物还原中使用乙醇作为能源进行 NAD(P)H 再生”发酵与生物工程杂志。