Improvement of blood compatibility and inorganic phosphate permeability by cationization of cellulosic dialysis membrane.

研究了多胺接枝、Michael反应和Hofmann降解对纤维素膜阳离子化的影响。研究了这些膜的血液相容性和无机磷酸盐的渗透性。以聚烯丙胺（PAAn）和六亚甲基二异氰酸酯（HMDI）为多胺和交联剂进行多胺接枝阳离子化。在30℃的温度下，HMDI在1小时内合成约0.02 μ mol/cm^2。PAAn与脲烷化膜在室温下容易发生反应。用二甲基亚砜代替甲苯，提高了阳离子化程度。通过改变反应条件和PAAn的分子量，可以制备出具有不同电位的阳离子膜。以二甲氨基丙基丙烯酰胺（DMAPAA）及其季胺化合物（TMAPAA）为原料，在45℃的温度下合成了0.34 μ mol/cm^2的DMAPAA和0.8 μ mol/cm^2的TMAPAA。在这些膜中观察到无机磷酸盐透析性能的改善。对氨基乙基纤维素膜进行了Hofmann降解，得到了0.094 μ mol/cm^2的氨基乙基化膜。在温和碱性条件下，尝试用乙二胺（EDA）或聚烯丙胺（PAAn）与n -氯化膜反应制备阳离子膜。EDA复合膜和PAAn复合膜的氨基酸含量分别为0.054 mu mol/cm^2和0.229 mu mol/cm^2。本研究中获得的几乎所有阳离子膜都显示出补体活化的抑制作用。

Cationization of cellulosic membranes by polyamine grafting, Michael reaction and Hofmann degradation was studied. Blood compatibility and inorganic phosphate permiability of these membranes were also studied.Polyallylamine(PAAn) and hexamethylene diisocyanate(HMDI) were used as the polyamine and the crosslinking agent for the cationization by polyamine grafting. About 0.02 mu mol/cm^2 of HMDI was combined within one hour reaction in toluene at 30ﾟC.The reaction of PAAn and urethanated membrane readily took place at room temperature. Degree of cationization was enhanced by using DMSO as a solvent instead of toluene. Cationic membranes of various zeta-potentials could be prepared by changing the reaction condition and the molecular weight of PAAn.Michael reaction using dimethylaminopropylacrylamide(DMAPAA) and its quaternary amine compound(TMAPAA) proceeded favorably, and 0.34 mu mol/cm^2 of DMAPAA and 0.8 mu mol/cm^2 of TMAPAA were combined at 45ﾟC.Improvement of the inorganic phosphate dialyzance was observed in these membranes.Hofmann degradation of carbamoylethylated cellulose membrane readily proceeded and aminoethylated membrane of 0.094 mu mol/cm^2 was obtained. Ethylenediamine(EDA) or polyallylamine(PAAn) was attempted to react with N-chlorinated membrane to prepare cationic membranes under a mild alkaline condition. The amino group content was 0.054 mu mol/cm^2 and 0.229 mu mol/cm^2 for EDA combined membrane and PAAn combined membrane, respectively. Almost all cationic membranes obtained in this study showed the suppression effect of complement activation from the CH50 measurement.