Improvement of blood compatibility and inorganic phosphate permeability by cationization of cellulosic dialysis membrane.

研究了纤维素膜的多胺接枝、Michael反应和Hofmann降解阳离子化。以聚烯丙基胺（PAAn）和二异氰酸酯（HMDI）为多胺和交联剂，通过多胺接枝阳离子化，制备了聚烯丙基胺（PAAn）/HMDI复合膜。在30 ℃下，在甲苯中反应1小时内，约0.02 μ mol/cm^2的HMDI结合。PAAn和交联膜的反应在室温下很容易发生。用DMSO代替甲苯作溶剂，提高了阳离子化程度。通过改变反应条件和PAAn的分子量，可以制得不同zeta电位的阳离子膜，以二甲基氨丙基丙烯酰胺（DMAPAA）及其季胺化合物（TMAPAA）为原料，0.34 μ mol/cm ^2的DMAPAA和0.8 μ mol/cm^2的DMAPAA。在45 ℃下，将2的TMAPAA组合在一起，在这些膜中观察到无机磷酸盐透析率的改善。氨甲酰乙基化纤维素膜容易进行霍夫曼降解，得到0.094 μ mol/cm ^2的氨乙基化膜。在弱碱性条件下，用乙二胺（EDA）或聚烯丙基胺（PAAn）与N-氯化膜反应制备阳离子膜。EDA组合膜和PAAn组合膜的氨基含量分别为0.054 μ mol/cm ^2和0.229 μ mol/cm ^2。本研究中获得的几乎所有阳离子膜均显示出来自CH 50测量的补体激活的抑制作用。

Cationization of cellulosic membranes by polyamine grafting, Michael reaction and Hofmann degradation was studied. Blood compatibility and inorganic phosphate permiability of these membranes were also studied.Polyallylamine(PAAn) and hexamethylene diisocyanate(HMDI) were used as the polyamine and the crosslinking agent for the cationization by polyamine grafting. About 0.02 mu mol/cm^2 of HMDI was combined within one hour reaction in toluene at 30ﾟC.The reaction of PAAn and urethanated membrane readily took place at room temperature. Degree of cationization was enhanced by using DMSO as a solvent instead of toluene. Cationic membranes of various zeta-potentials could be prepared by changing the reaction condition and the molecular weight of PAAn.Michael reaction using dimethylaminopropylacrylamide(DMAPAA) and its quaternary amine compound(TMAPAA) proceeded favorably, and 0.34 mu mol/cm^2 of DMAPAA and 0.8 mu mol/cm^2 of TMAPAA were combined at 45ﾟC.Improvement of the inorganic phosphate dialyzance was observed in these membranes.Hofmann degradation of carbamoylethylated cellulose membrane readily proceeded and aminoethylated membrane of 0.094 mu mol/cm^2 was obtained. Ethylenediamine(EDA) or polyallylamine(PAAn) was attempted to react with N-chlorinated membrane to prepare cationic membranes under a mild alkaline condition. The amino group content was 0.054 mu mol/cm^2 and 0.229 mu mol/cm^2 for EDA combined membrane and PAAn combined membrane, respectively. Almost all cationic membranes obtained in this study showed the suppression effect of complement activation from the CH50 measurement.