Improvement of blood compatibility and inorganic phosphate permeability by cationization of cellulosic dialysis membrane.

研究了多胺接枝、Michael反应和Hofmann降解对纤维素膜的阳离子作用。研究了聚烯丙胺(PAAN)和六亚甲基二异氰酸酯(HMDI)作为多胺接枝阳离子的交联剂和聚烯丙胺(PAAN)膜的血液相容性和无机磷渗透性能。在30ﾟC的甲苯溶液中，约0.02mU·m o l/cm~2的HMDI在1小时内反应。PAAN与氨酯膜的反应在室温下很容易发生。用DMSO代替甲苯作溶剂，提高了阳离子化程度。通过改变反应条件和PAN的相对分子质量，可以制得不同zeta电位的阳离子膜。二甲氨基丙基丙烯酰胺与其季胺化合物的Michael反应顺利进行，在45ﾟC时，DMAPAA和TMAPAA的摩尔浓度分别为0.34mU/cm~2和0.8mU/cm~2，膜的无机磷酸盐透析力得到改善。尝试了乙二胺(EDA)或聚烯丙基胺(PAAN)在弱碱性条件下与N-氯化膜反应制备阳离子膜。乙二胺复合膜和PAAN复合膜的氨基含量分别为0.054 mU/cm2和0.229 mU/cm2。几乎所有在本研究中获得的阳离子膜都从CH50的测量中显示出对补体激活的抑制作用。

Cationization of cellulosic membranes by polyamine grafting, Michael reaction and Hofmann degradation was studied. Blood compatibility and inorganic phosphate permiability of these membranes were also studied.Polyallylamine(PAAn) and hexamethylene diisocyanate(HMDI) were used as the polyamine and the crosslinking agent for the cationization by polyamine grafting. About 0.02 mu mol/cm^2 of HMDI was combined within one hour reaction in toluene at 30ﾟC.The reaction of PAAn and urethanated membrane readily took place at room temperature. Degree of cationization was enhanced by using DMSO as a solvent instead of toluene. Cationic membranes of various zeta-potentials could be prepared by changing the reaction condition and the molecular weight of PAAn.Michael reaction using dimethylaminopropylacrylamide(DMAPAA) and its quaternary amine compound(TMAPAA) proceeded favorably, and 0.34 mu mol/cm^2 of DMAPAA and 0.8 mu mol/cm^2 of TMAPAA were combined at 45ﾟC.Improvement of the inorganic phosphate dialyzance was observed in these membranes.Hofmann degradation of carbamoylethylated cellulose membrane readily proceeded and aminoethylated membrane of 0.094 mu mol/cm^2 was obtained. Ethylenediamine(EDA) or polyallylamine(PAAn) was attempted to react with N-chlorinated membrane to prepare cationic membranes under a mild alkaline condition. The amino group content was 0.054 mu mol/cm^2 and 0.229 mu mol/cm^2 for EDA combined membrane and PAAn combined membrane, respectively. Almost all cationic membranes obtained in this study showed the suppression effect of complement activation from the CH50 measurement.