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Studies on Placental Transfer and Milk Secretion of Zn-65 and Mn-54 in Experimental Animals

Zn-65、Mn-54在实验动物胎盘移植及乳汁分泌的研究

基本信息

批准号：
04660316
负责人：
MATSUSAKA Naonori
金额：
$ 1.34万
依托单位：
IWATE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The fetal uptake of radioactive zinc (Zn-65) and manganese (Mn-54) was studied as a function of gestational age in mice. The fetal uptake of both nuclides increased markedly with time after administration to the pregnant mice of gestation Day 17, i.e., from 3% of the dose 30 min after administration to 36% of the dose 24 hr after administration as a litter. The similar increasing tendency of the fetal uptake of Mn-54 was observed in mice of the same gestatonal age, i.e., from 3% of the dose 30 min after administration to 9% of the dose 24 hr after administration as a litter. The fetal uptake of Zn-65 24 hr after administration was 4 times greater than that of Mn-54.These two nuclides were given simultaneously with a single subcutaneous dose to the pregnant mice of gestation Day 0, 7 and 14 as a double tracer, and the radioactivity in newborns just after birth was measured by a pulse-height analyzer. The amounts of Zu-65 transferred to fetuses in utero in the pregnant mice of gestation Day 0, 7 and 14 were 11%, 12% and 28% of the dose as a litter, respectively. Therefore, the amount of Zu-65 in the newborns became gerater when the gestation period advanced to tern. Similar results were obtained concerning Mn-54.The amount of Zn-65 and MN-54 secreted into milk was estimated from the pup's body burden during nursing period, using foster-dam method. It was demonstrated that the secretion of both nuclides into milk is an imnportant pathway of elimination.

研究了胎儿对放射性锌 (Zn-65) 和锰 (Mn-54) 的吸收与小鼠胎龄的函数关系。妊娠第 17 天的怀孕小鼠给药后，胎儿对两种核素的摄取量随着时间的推移而显着增加，即从给药后 30 分钟剂量的 3% 增加到给药后 24 小时剂量的 36%。在相同胎龄的小鼠中观察到胎儿对 Mn-54 摄取的类似增加趋势，即从给药后 30 分钟剂量的 3% 到给药后 24 小时剂量的 9%。给药后24小时胎儿对Zn-65的摄取量是Mn-54的4倍。将这两种核素同时单次皮下注射给妊娠第0、7和14天的怀孕小鼠作为双重示踪剂，并用脉冲高度分析仪测量刚出生的新生儿的放射性。在妊娠第0、7和14天的怀孕小鼠中，Zu-65转移到子宫内胎儿的量分别是窝剂量的11%、12%和28%。因此，当妊娠期提前到燕鸥时，新生儿体内Zu-65的含量变得更高。 Mn-54也得到了类似的结果。利用寄养法，根据幼犬在哺乳期间的身体负担估算了分泌到乳汁中的Zn-65和MN-54的量。研究表明，两种核素分泌到乳汁中是重要的消除途径。