CELLULAR DIFFERENTIATION OF HEART AND VESSEL

心脏和血管的细胞分化

• 批准号: 04670025
• 负责人: NAKAJIMA Yuji
• 金额: $ 1.34万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670025/
• 关键词: Precardiac mesoderm; Cardiomyocyte; Differentiation; Growth factor; Chick embryo; Transposition of the great arteries; Retinoic acid; Mouse embryo; 筋小胞体; 形質転換成長因子; 心臓; 血管; 発生; 細胞分化; 体外培養; 平滑筋細胞; 内皮細胞

1.Precardiac mesoderm and growth factorsPrecardiac mesoderm, which has potential ability of differentiation to beating myocardium in the adult heart, was examined whether it developed to beating tissue in the presence of growth factors. Insulin-like growth factor, activin A,and basic fibroblast growth factor showed a stimulating effect on myocardial differentiation.2.Heart development and calcium-containing mitochondriaA calcium-containing vacuolated mitochondria in chick developing heart was examined by cytochemistry and X-ray microanarysis. Results suggested that vacuolated mitochondria regulated intracellular calcium concentration.3.Sarcoplasmic reticulum and heart developmentDevelopment of sarcoplasmic reticulum (SR) and its calcium releasing function were examined in early chick embryonic heart. Tubular SR and junctional SR appeared at stage 11 and 12, respectively. Intracellular calcium concentration by fura-2 suggested that SR might elicit its function at stage 10.4.Transposition of the great arteries (TGA)Developmental mechanism of TGA,one of the congenital heart malformations, was examined in retinoic-acid induced mouse model. Intraracardiac cast models at various developmental stages were examined by scanning electron microscopy. Primary morphological abnormality of TGA was hypoplasia of cushion ridges in conus cordis.5.Transforming growth factor (TGF) BETA3 and chick embryogenesismRNA transcripts of TGFBETA3 was examined in developing chick embryos by in situ hybridization. TGFBETA3 mRNA was distributed mainly at developing artery and heart as well as epithelium of body cavity. This suggested that TGFBETA3 may play important roles in mesodermal development including cardiovascular system.

1.心前中胚层与生长因子检测在生长因子存在下，具有向心脏搏动心肌分化潜能的心前中胚层是否发育为搏动组织。胰岛素样生长因子、激活素A和碱性成纤维细胞生长因子对心肌分化有促进作用。2.心脏发育和含钙线粒体A细胞化学和X射线微核分析。3.肌浆网与心脏发育观察早期鸡胚胎心脏肌浆网的发育及其钙释放功能。管状性和连接性SR分别出现在第11期和第12期。Fura-2检测细胞内钙离子浓度提示SR可能在10.4期诱导其功能。大动脉移位(TGA)是先天性心脏畸形之一。用扫描电子显微镜观察不同发育阶段的心腔内铸型模型。TGA的主要形态异常是圆锥体垫脊发育不全。5.用原位杂交技术检测转化生长因子β3和鸡胚胎发生中TGFBETA3的RNA转录本。TGFBETA3mRNA主要分布于发育中的动脉、心脏及体腔上皮细胞。提示TGFBETA3可能在包括心血管系统在内的中胚层发育中起重要作用。

项目成果

中島裕司: "Annual Review 循環器1994" 中外医学社 青木三千雄, 2 (1994)

中岛雄二：《心脏病学年度回顾 1994》中外医学社青木道雄，2 (1994)

Yoshihito Yamazaki: "Effect of growth factors on the differentiation of chick precardiac mesoderm in vitro" Roux's Archives of Developmental Biology. (in press).

Yoshihito Yamazaki：“生长因子对体外鸡心前中胚层分化的影响”Roux 发育生物学档案。

Tamiko, Hiruma: "Formation of the pharyngeal arch arteries in the chick embryo. Observations of corrosion casts by scanning electron microscopy." Anatomy and Embryology. 191(in Press). (1995)

Tamiko, Hiruma：“鸡胚胎中咽弓动脉的形成。通过扫描电子显微镜观察腐蚀铸件。”

TAMIKO HIRUMA: "FORMATION OF THE PHARYNGEAL ARCH ARTERIES IN THE CHICK EMBRYO." OBSERVATION OF CORROSION CASTS BY SCANNING ELECTRON MICROSCOPY.ANAT.EMBRYOL.191 (IN PRESS). (1995)

TAMIKO HIRUMA：“鸡胚胎中咽弓动脉的形成。”

Shinji Komazaki: "Calcium-containing vacuolated mitochondria during early heart development in chick embryos as demonstrated by cytochemistry and X-ray microanalysis" Anatomy and Embryology. 189. 441-446 (1994)

Shinji Komazaki：“通过细胞化学和 X 射线微量分析证明了鸡胚胎早期心脏发育过程中的含钙空泡线粒体”《解剖学与胚胎学》。