Exploration of novel mechanisms regulating early cardiomyocyte development by using a differential-display method

利用差异显示方法探索调节早期心肌细胞发育的新机制

• 批准号: 20390052
• 负责人: NAKAJIMA Yuji
• 金额: $ 7.07万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390052/
• 关键词: 発生学・形態形成学; 中胚葉; 細胞移動; 解剖学; 発生・分化; 原腸陥入; 心臓; 心筋; 細胞運動

During axis formation in amniotes, posterior and lateral epiblast cells in the area pellucida undergo a counter-rotating movement along the midline to formprimitive streak (Polonaise movements). Using chick blastoderms, we investigated thesignaling involved in this cellular movement in epithelial-epiblast. In cultured posteriorblastoderm explants from stage X-XI embryos, either Lefty1 or Cerberus-S inhibited initialmigration of the explants on chamber slides. In vivo analysis showed that inhibition ofNodal signaling by Lefty1 affected the movement of DiI-marked epiblast cells prior to theformation of primitive streak. In Lefty1-treated embryos without a primitive streak,Brachyury expression showed a patchy distribution. However, SU5402 did not affect themovement of DiI-marked epiblast cells. Multi-cellular rosette, which is thought to beinvolved in epithelial morphogenesis, was found predominantly in the posterior half of theepiblast, and Lefty1 inhibited the formation of rosettes. Three-dimensional reconstruction showed two types of rosette, one with a protruding cell, the other with a ventral hollow.Our results suggest that Nodal signaling may have a pivotal role in the morphogeneticmovements of epithelial epiblast including Polonaise movements and formation of multi-cellular rosette.

在羊膜轴形成过程中，透明区的后外侧外胚层细胞沿着中线进行反向旋转运动，形成原始条纹（Polonaise运动）。利用鸡胚皮，我们研究了上皮-外胚层中参与这种细胞运动的信号传导。在培养的X-XI期胚后胚层外植体中，Lefty1或Cerberus-S均能抑制外植体在载玻片上的初始迁移。体内分析表明，在原始条纹形成之前，Lefty1对节点信号的抑制影响了dii标记的外胚层细胞的运动。在没有原始条纹的lefty1处理的胚胎中，Brachyury表达呈斑片状分布。然而，SU5402不影响dii标记的外胚层细胞的运动。多细胞莲座被认为参与上皮细胞的形态发生，主要存在于外胚层的后半部分，而Lefty1抑制了莲座的形成。三维重建显示了两种类型的玫瑰花结，一种是突出的细胞，另一种是腹侧中空的细胞。我们的研究结果表明，节点信号可能在上皮外胚层的形态发生运动中起关键作用，包括Polonaise运动和多细胞玫瑰花结的形成。

项目成果

Immunohistochemical studies of aortic smooth muscle cells in quail embryo.

鹌鹑胚胎主动脉平滑肌细胞的免疫组织化学研究。

• 发表时间: 2011
• 作者: Ando K;Nakajima Y;Yamagishi T;Nakamura H.
• 通讯作者: Nakamura H.

ニワトリ胞胚期から原腸期に起こる予定心臓細胞の遊走

预期心肌细胞从鸡囊胚期向原肠胚期的迁移

• 发表时间: 2008
• 作者: 柳川成章;坂田大和;坂部正英;中島裕司
• 通讯作者: 中島裕司

Morphological and molecular dissection of retinoic acid (RA)-induced transposition of the great arteries.

视黄酸（RA）诱导的大动脉转位的形态学和分子解剖。

• 发表时间: 2011
• 作者: Tanaka S;Sakamoto K;Yamamoto M;Mizuno A;Ono T;Waguri S;Kimura J.;Nakajima Y.
• 通讯作者: Nakajima Y.

Inhibition of Nodal signaling affects epiblast cell movement in the pre-streak chick blastoderm.

抑制 Nodal 信号传导会影响前条纹雏鸡胚层中的外胚层细胞运动。

• 发表时间: 2009
• 作者: Yanagawa N;Yamagishi T;Nakajima Y.
• 通讯作者: Nakajima Y.

Role of sox9 during valvulo-septal endocardial cushion formation.

sox9 在瓣膜间隔心内膜垫形成过程中的作用。

• 发表时间: 2011
• 作者: Yamagishi Y;Yanagawa N;Takahashi M;Ando K;Nakamura H;Nakajima Y.
• 通讯作者: Nakajima Y.