Mechanism of Ca^<2+> oscillation in salivaly gland acinar cells

唾液腺腺泡细胞Ca^<2>振荡机制

• 批准号: 04670044
• 负责人: WAKUI Makoto
• 金额: $ 1.09万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670044/
• 关键词: Ca^<2+> oscillation; Ca^<2+> wave; Ca^<2+> release; Ca^<2+> entry; inositol trisphosphate; IP_3 receptor; acetylcholine; exocrine gland cells; カルシウムイオン; カルシウム振動; ケージド物質; 顎下腺腺房細胞; イオン性膜電流; パッチクランプ法; 細胞内カルシウム; Ca^<2+>依存性膜電流; 細胞内Ca^<2+>振動; ケ

Generation of the Ca^<2+> oscillation in response to secretagogues in exocrine gland acinar cells depends on two Ca^<2+> mobilizing mechanisms ; Ca^<2+> release from intracellular stores and Ca^<2+> entry from the extracellular space. To explore the precise mechanism of Ca^<2+> release, caged inositol trisphosphate (IP_3) was employed into the submandibular cells, and both Ca^<2+> activated Cl^- and K^+ currents were recorded. Photolytic transient release of a small amount of IP_3 caused only transient outward K^+ and inward Cl^- current, both of which were activated by Ca^<2+> released. On the other hand, release of relatively large amount of IP^3 caused additional Ca^<2+> activated delayd outward current. These indicate that Ca^<2+> releases from the IP_3-sensitive pools in an IP_3 concentration-dependen manner. Low concentrations of IP_3 release Ca^<2+> only locally, while high concentrations of IP_3 induce additinal Ca^<2+> release, which may be refect the Ca^<2+> wave progagation. … More The role of extracellular Ca^<2+> in the Ca^<2+> oscillation induced by acetylcholine (A Ch) was further studied by recording the Ca^<2+>-activated Cl^- currents. The higher the concentration of extracellular concentration of Ca^<2+>, the larger the amplitude of the repetitive Cl^- currents recorded. The Ca^<2+> oscillation ceased after removal of Ca^<2+> from the extracellular solution, and application of Ca^<2+> restored the Ca^<2+> oscillations. Application of Ca^<2+> to the extracellular solution after depletion of Ca^<2+> store by the first A Ch stimulation did not show any change in the membrane current in a condition without A Ch. However, the second stimulation with A Ch caused repetitive Ca^<2+>-activated Cl^- currents in a condition of Ca^<2+> being removed from the extracellular solution. These results indicate that the Ca^<2+> entry is essential to maintain the repetitive release of Ca^<2+>. Results further suggest that Ca^<2+> entering the cell is immediately taken up by Ca^<2+> stores and released by IP_3. Less

响应于外分泌腺腺泡细胞中的促分泌物的Ca^<2+>振荡的产生取决于两个Ca^<2+>动员机制； Ca^<2+>从细胞内存储中释放，Ca^<2+>从细胞外空间进入。为了探索Ca^<2+>释放的精确机理，将笼中的肌醇三磷酸（IP_3）携带到下颌细胞中，并记录了Ca^<2+>激活的Cl^ - 和K^+电流。少量IP_3的光解瞬态释放仅引起瞬态向外k^+和内向cl^ - 电流，这两者都被Ca^<2+>释放而激活。另一方面，释放相对较大的IP^3导致额外的Ca^<2+>激活的向外电流延迟。这些表明Ca^<2+>以IP_3浓度依赖性方式从IP_3敏感池释放。低浓度的IP_3释放Ca^<2+>仅在本地释放，而高浓度的IP_3影响附加的Ca^<2+>释放，这可能会损坏Ca^<2+>波进程。 …更多通过记录Ca^<2+> - 激活的Cl^ - 电流，进一步研究了乙酰胆碱（A CH）诱导的Ca^<2+>振荡中的作用。 Ca^<2+>的细胞外浓度的浓度越高，记录的重复Cl^ - 电流的放大器越大。从细胞外溶液中去除Ca^<2+>后，CA^<2+>振荡停止，并且应用Ca^<2+>恢复了Ca^<2+>振荡。在耗尽Ca^<2+>储存后，将Ca^<2+>施加到细胞外溶液中，第一个A CH刺激在没有CH的情况下没有显示出膜电流的任何变化。但是，用CH的第二个刺激导致重复的Ca^<2+> - 激活的Cl^ - 在Ca^<2+>的条件下被从细胞外溶液中取出。这些结果表明，CA^<2+>进入对于维持Ca^<2+>的重复释放至关重要。结果进一步表明，Ca^<2+>进入该单元格的结果立即被CA^<2+>商店占据，并由IP_3释放。较少的

项目成果

W.ZHANG: "Role of extracellular Ca^<2+> in acetylcholine-induced repetitive Ca^<2+> release in submandibular gland acinar cells of the rat." J.Cell.Physiol.167. 277-284 (1996)

W.ZHANG：“细胞外Ca^2在乙酰胆碱诱导的大鼠颌下腺腺泡细胞重复Ca^2释放中的作用”。

Wakui M,Wada J,Kamimura N,Mio Y,Sasaki T,Fukushi Y,and Nishiyama A: "Ca^<2+> entry through the store-mediated pathway directly activates only the K^+ current but the subsequent Ca^<2+> release from the store activates both K^+ and Cl^- currents in submand

Wakui M、Wada J、Kamimura N、Mio Y、Sasaki T、Fukushi Y 和 Nishiyama A：“Ca^<2> 通过存储介导途径进入直接仅激活 K^ 电流，但随后的 Ca^<2>

Sasaki T,Shimura S,Wakui M,Ohkawara Y,Takishima T,and Mikoshiba K: "Apically localized IP_3 receptors control chloride current in airway gland acinar cells" Am.J.Physiol.267. L152-L158 (1994)

Sasaki T、Shimura S、Wakui M、Ohkawara Y、Takishima T 和 Mikoshiba K：“顶部局部 IP_3 受体控制气道腺腺泡细胞中的氯电流”Am.J.Physiol.267。

Wakui,M. et al.: "Sustained and transient calcium release from intracellular calcium pools involved in IP_3-induced responses in rat submandibular gland acinar cells." 32th Congress of The International Union of Physiological Science. 160 (1993)

和奎，M.

M.WAKUI: "Ca^<2+> entry through the store-mediated pathway directly activates only the K^+ current but the subsequent Ca^<2+> release from the store activates both K^+ and Cl-currents in submandibular gland acinar cells of the rat." Cell.Signalling. 7. 78

M.WAKUI：“Ca^<2> 通过存储介导的途径进入直接仅激活 K^ 电流，但随后从存储中释放的 Ca^<2> 会激活下颌下腺腺泡细胞中的 K^ 和 Cl 电流。