Mechanism of Ca^<2+> oscillation in salivaly gland acinar cells

唾液腺腺泡细胞Ca^<2>振荡机制

• 批准号: 04670044
• 负责人: WAKUI Makoto
• 金额: $ 1.09万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670044/
• 关键词: Ca^<2+> oscillation; Ca^<2+> wave; Ca^<2+> release; Ca^<2+> entry; inositol trisphosphate; IP_3 receptor; acetylcholine; exocrine gland cells; カルシウムイオン; カルシウム振動; ケージド物質; 顎下腺腺房細胞; イオン性膜電流; パッチクランプ法; 細胞内カルシウム; Ca^<2+>依存性膜電流; 細胞内Ca^<2+>振動; ケ

Generation of the Ca^<2+> oscillation in response to secretagogues in exocrine gland acinar cells depends on two Ca^<2+> mobilizing mechanisms ; Ca^<2+> release from intracellular stores and Ca^<2+> entry from the extracellular space. To explore the precise mechanism of Ca^<2+> release, caged inositol trisphosphate (IP_3) was employed into the submandibular cells, and both Ca^<2+> activated Cl^- and K^+ currents were recorded. Photolytic transient release of a small amount of IP_3 caused only transient outward K^+ and inward Cl^- current, both of which were activated by Ca^<2+> released. On the other hand, release of relatively large amount of IP^3 caused additional Ca^<2+> activated delayd outward current. These indicate that Ca^<2+> releases from the IP_3-sensitive pools in an IP_3 concentration-dependen manner. Low concentrations of IP_3 release Ca^<2+> only locally, while high concentrations of IP_3 induce additinal Ca^<2+> release, which may be refect the Ca^<2+> wave progagation. … More The role of extracellular Ca^<2+> in the Ca^<2+> oscillation induced by acetylcholine (A Ch) was further studied by recording the Ca^<2+>-activated Cl^- currents. The higher the concentration of extracellular concentration of Ca^<2+>, the larger the amplitude of the repetitive Cl^- currents recorded. The Ca^<2+> oscillation ceased after removal of Ca^<2+> from the extracellular solution, and application of Ca^<2+> restored the Ca^<2+> oscillations. Application of Ca^<2+> to the extracellular solution after depletion of Ca^<2+> store by the first A Ch stimulation did not show any change in the membrane current in a condition without A Ch. However, the second stimulation with A Ch caused repetitive Ca^<2+>-activated Cl^- currents in a condition of Ca^<2+> being removed from the extracellular solution. These results indicate that the Ca^<2+> entry is essential to maintain the repetitive release of Ca^<2+>. Results further suggest that Ca^<2+> entering the cell is immediately taken up by Ca^<2+> stores and released by IP_3. Less

外分泌腺腺泡细胞中促分泌素引起的Ca^2+振荡依赖于两种Ca^2+动员机制：细胞内储存的Ca^2+释放和细胞外间隙的Ca^2+进入。为探讨Ca^2+释放的确切机制，将笼状三磷酸肌醇（IP_3）作用于下颌下神经细胞，记录Ca^2+激活的Cl^-和K^+电流。少量IP_3的光解瞬时释放仅引起瞬时外向K^+和内向Cl^-电流，这两种电流都被释放的Ca^<2+>激活。另一方面，大量释放IP^3引起额外的Ca^2+激活的延迟外向电流。这表明IP_3浓度依赖性地从IP_3敏感池中释放Ca^<2+>。低浓度IP_3仅局部释放Ca^<2+>，而高浓度IP_3则诱导额外的Ca^<2+>释放，这可能反映了Ca^<2+>波的衰减。 ...更多信息 通过记录细胞外Ca^<2 +>激活的Cl^-电流进一步研究了细胞外Ca^<2 +>在乙酰胆碱（ACh）诱导的Ca^<2+>振荡中的作用。细胞外Ca^<2+>浓度越高，记录到的重复Cl^-电流幅度越大。从细胞外溶液中去除Ca^<2+>后，Ca^<2 +>振荡停止，而Ca^<2+>的应用恢复了Ca^<2+>振荡。在没有ACh的条件下，在第一次刺激耗尽Ca^2+储存后，将Ca^2+施加到细胞外溶液中，没有显示出膜电流的任何变化。然而，在从细胞外溶液中去除Ca^2+的条件下，用ACh进行的第二次刺激引起了重复的Ca^2+激活的Cl^-电流。这些结果表明，Ca^2+内流对维持Ca^2+的重复释放是必不可少的。结果进一步表明，进入细胞的Ca^<2+>立即被Ca^<2+>库吸收，并由IP_3释放。少

项目成果

W.ZHANG: "Role of extracellular Ca^<2+> in acetylcholine-induced repetitive Ca^<2+> release in submandibular gland acinar cells of the rat." J.Cell.Physiol.167. 277-284 (1996)

W.ZHANG：“细胞外Ca^2在乙酰胆碱诱导的大鼠颌下腺腺泡细胞重复Ca^2释放中的作用”。

Sasaki T,Shimura S,Wakui M,Ohkawara Y,Takishima T,and Mikoshiba K: "Apically localized IP_3 receptors control chloride current in airway gland acinar cells" Am.J.Physiol.267. L152-L158 (1994)

Sasaki T、Shimura S、Wakui M、Ohkawara Y、Takishima T 和 Mikoshiba K：“顶部局部 IP_3 受体控制气道腺腺泡细胞中的氯电流”Am.J.Physiol.267。

Wakui M,Wada J,Kamimura N,Mio Y,Sasaki T,Fukushi Y,and Nishiyama A: "Ca^<2+> entry through the store-mediated pathway directly activates only the K^+ current but the subsequent Ca^<2+> release from the store activates both K^+ and Cl^- currents in submand

Wakui M、Wada J、Kamimura N、Mio Y、Sasaki T、Fukushi Y 和 Nishiyama A：“Ca^<2> 通过存储介导途径进入直接仅激活 K^ 电流，但随后的 Ca^<2>

M.WAKUI: "Ca^<2+> entry through the store-mediated pathway directly activates only the K^+ current but the subsequent Ca^<2+> release from the store activates both K^+ and Cl-currents in submandibular gland acinar cells of the rat." Cell.Signalling. 7. 78

M.WAKUI：“Ca^<2> 通过存储介导的途径进入直接仅激活 K^ 电流，但随后从存储中释放的 Ca^<2> 会激活下颌下腺腺泡细胞中的 K^ 和 Cl 电流。

Wakui,M. et al.: "Sustained and transient calcium release from intracellular calcium pools involved in IP_3-induced responses in rat submandibular gland acinar cells." 32th Congress of The International Union of Physiological Science. 160 (1993)

和奎，M.