MOLECULAR STUDY ON CYTOTOXIN OF PSUDO-MONAS AERUGINOSA AND CYTOTOXIN-CONVERTING PHAGE

铜绿假单胞菌细胞毒素及细胞毒素转化噬菌体的分子研究

• 批准号: 04670243
• 负责人: MATSUMOTO Hideki
• 金额: $ 1.41万
• 依托单位: DEPARTMENT OF BACTERIOLOGY, SCHOOL OF MEDICINE, SHINSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670243/
• 关键词: Pseudomonas aeruginosa; Bacteriophage; Cytotoxin; attachment site; cos site; tRNA gene; R-pyocin; LPS; バクテリアファージ; バクテリオファージ; LPS

1.Molecular analysis of phage PHICTX : (1)Structure of cos site has been determined. (2)Structure of the "core" region of the attB site has been clarified. The attB of the PHICTX was found to be the 3' region of serine-tRNA gene on chromosome which was lcated 42'-45' region of genetic map of P.aeruginosa PAO.The region has a significant homology, in its nucleotide sequence, with the corresponding region of E.coli. (3)Existence of many "repeated sequences", "bent-DNA" and the binding sequence for IHF has become evident in the att-cos-ctx region. (4)The two genes, which were supposed to be the int and xis, respectively, of PHICTX have been found near the attP.2.Relationship between PHICTX and R(Rod shaped)Pyocins : (1)PHICTX has been identified as an R Pyocin-related phage based on immunological property and homology of DNA sequences. (2)Receptor of PHICTX has been identified as the outer-core region of LPS.Infectivity of PHICTX confined only a restricted serotypes. (3)Among the R pyocin-related phages tested, PS21 was proved to have ctx. (4)Distribution of the R pyocin-related phages without ctx but have homology with PHICTX genome was confirmed. 3.Search for the genes related to ctx in other bacteria : This has been done for several hundred strains of various bacterial species using DNA of ctx as the probe by Southern test but none of gene hybridized with the probe.

1.噬菌体PHICTX的分子分析：(1)确定了cos位点的结构。(2)阐明了attB位点“核心”区域的结构。PHICTX的attB基因是位于铜绿假单胞菌PAO遗传图谱42‘-45’区域的丝氨酸-tRNA基因的3‘端区域，该区域的核苷酸序列与大肠杆菌的相应区域有显著的同源性。(3)At-cos-CTX区存在许多“重复序列”、“弯曲DNA”和与IHF结合的序列。(4)在AttP附近发现了PHICTX的两个基因，分别为int和Xis。2.PHICTX与R(杆状)噬菌素的关系：(1)根据DNA序列的同源性和免疫学性质，PHICTX被鉴定为与R毒素相关的噬菌体。(2)PHICTX的受体是LPS的外核区，其感染性仅限于有限的血清型。(3)在所检测的R噬菌素相关噬菌体中，PS21被证明含有CTX。(4)证实了不含CTX但与PHICTX基因组同源的R噬菌素相关噬菌体的分布。3.在其他细菌中寻找与CTX相关的基因：用CTX的DNA作为探针，对数百株不同菌种的细菌进行了Southern检测，但没有一个基因与该探针杂交。

项目成果

HAYASHI,T.: "Pseudomonas aeruginosa cytotoxin and its phage conversion.(in Japanese)" Nippon Nogeikagaku Kaishi. 68(2). 259-262 (1994)

HAYASHI,T.：“铜绿假单胞菌细胞毒素及其噬菌体转化。（日语）”Nippon Nogeikagaku Kaishi。

林 哲也: "緑膿菌サイトトキシンとそのファージ変換" 日本農芸化学会誌. 68. 259-262 (1994)

Tetsuya Hayashi：“铜绿假单胞菌细胞毒素及其噬菌体转化”日本农业化学学会杂志 68. 259-262 (1994)。

T.Hayashi,H.Matsumatsumoto,M.Ohonishi,Y.Terawaki: "Molecular characterization of a cytotoxin-converting phage,φCTX,of Pseudomonas aeruginosa:structre of the attP-cos-ctx region and integration int Lheserine t RNA gene." Molecular Microbiology,. 7(5). (199

T. Hayashi、H. Matsumatsumoto、M. Ohonishi、Y. Terawaki：“铜绿假单胞菌细胞毒素转化噬菌体 φCTX 的分子特征：attP-cos-ctx 区域的结构和 Lheserine t RNA 基因的整合。”分子微生物学，7(5)。

Hayashi,T.,Matsumoto,H.,Ohnishi,M.and Terawaki,Y.: "Molecular analysis of a cytotoxin-converting phage,oCTX,of Pseudomonas aeruginosa:structure of the attP-cos-ctx region and integration into the serine tRNA gene." Molecular Microbiology. 7. 657-667 (1993

Hayashi,T.、Matsumoto,H.、Ohnishi,M. 和 Terawaki,Y.：“铜绿假单胞菌细胞毒素转化噬菌体 oCTX 的分子分析：attP-cos-ctx 区域的结构以及与丝氨酸的整合

HAYASHI,T., MATSUMOTO,H., OHNISHI,M.AND TERAWAKI,Y.: "Molecular analysis of a cytotoxin-converting phage, PHICTX, of Pseudomonas aeruginosa : structure of the attp-cos-ctx region and integration into serine tRNA gene." Molecular Microbiology. 7(5). 657-66

HAYASHI,T.、MATSUMOTO,H.、OHNISHI,M. 和 TERAWAKI,Y.：“铜绿假单胞菌细胞毒素转化噬菌体 PHICTX 的分子分析：attp-cos-ctx 区域的结构以及与丝氨酸 tRNA 的整合