Molecular Cloning of Pancreas Ductal Antigen and the ELISA Method using Recombinant Protein, for a Diagnosis of ldiopathic Chronic Pancreatitis.

本项目的目的是确定单克隆抗体（SP3-1）识别的导管抗原（PA3）的性质。碳酸酐酶（CA）的分布与PA3基本相同。此外，用免疫印迹法检测到CA在部分分子中可能具有相同的氨基酸序列。因此，碳酸酐酶I （CA I）和碳酸酐酶II （CA II）可作为导管抗原评估干燥综合征（SjS）或特发性慢性胰腺炎（ICP）患者血清抗体水平。血清抗cai抗体的检测。对SjS和ICP患者血清进行酶联免疫吸附试验。SjS患者的血清数据显示，与正常志愿者或酒精性慢性胰腺炎（CP）加CP合并胆石症患者相比，SjS患者的血清有显著差异（P<0.05）。ICP患者血清数据显示，与正常志愿者、酒精性CP或与胆石症相关的CP相比，有显著差异（P<0.05）。血清抗caii抗体的检测。对SjS和ICP患者血清进行酶联免疫吸附试验。与正常志愿者相比，SjS患者的血清数据显示有显著差异（P<0.05）。与正常志愿者、酒精性CP或胆石症相关CP相比，ICP患者血清数据显示有显著差异（P<0.05）。结果表明CAⅱ对胰腺导管抗原的特异性高于CAⅰ。为证实这一点，有必要对SjS或ICP患者进行更多的免疫学检查和更多的血清检查。

The aim of this project is to make sure a property of duct antigen (PA3) which is recognized by monoclonal antibody (SP3-1). The distribution of Carbonic Anhydrase(CA) is almost same as PA3. Furthermore we detected CA may have same amino acid sequence in a part of molecule by Western Immunoblotting method. So Carbonic Anhydrase I (CA I ) and Carbonic Anhydrase II (CA II ) were available as a duct antigen to evaluate an antibody levels of sera from patients with Sjogren's syndrome(SjS) or Idiopathic Chronic Pancreatitis(ICP) in this research.1.Detection of Anti-CAI Antibody in Serum. Sera from patients with SjS and ICP were titered in the ELISA.Data on the sera from patients with SjS revealed significant difference (P<0.05), compared with normal volunteers or alcoholic chronic pancreatitis(CP) plus CP associated with cholelithiasis. Data on the sera from patients with ICP revealed significant difference (P<0.05), compared with normal volunteers or alcoholic CP or CP associated with cholelithiasis.2.Detection of Anti-CAII Antibody in Serum. Sera from patients with SjS and ICP were titered in the ELISA.Data on the sera from patients with SjS revealed significant difference (P<0.05), compared with only normal volunteers. Data on the sera from patients with ICP revealed significant difference (P<0.05), compared with normal volunteers or alcoholic CP or CP associated with cholelithiasis.This results show that CA II has more specificity than CA I in respect of pancreas ductal antigen. In order to make it sure, more immunological examination and check of more sera from patients with SjS o r ICP are necessary.