Creation of Novel Anti-allergic Leads Based on the Inhibition for Expression of IgE Receptors

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Allergy is caused by chemical mediators degranulated from mast cells. This degranulation is derived through the important two steps. In a first step, immunoglobulin Es (IgEs) are attached on the IgE receptors on the surface of mast cells. Then, the IgEs on mast cells react with invaded allergens in a second step. In this circumstance, I wondered that the decrease of IgE receptors on the mast cells may be able to suppress allergic reactions. Based on this idea, I established the assay system for evaluation of the amount of IgE receptors on the surface of cultured mast cells by immunofluorescence staining and flow cytometry. By the screening from medicinal plants for inhibitors against expression of IgE receptors, chlorin e6 was found as a potent inhibitor. Fortunately, chlorin e6 suppressed the expression of IgE receptors not only in vitro but also in vivo rat experiments. Finally, chlorin e6 was clarified to inhibit in vivo allergic reaction of rats through po administration.