Studies on the metabolism of thyroid tumor using Nuclear Medicine

早期和延迟扫描的T1摄取率半定量为早期(ER)和延迟率(DR)。同时用流式细胞术(FCM)检测细胞核DNA含量和增殖细胞核抗原(PCNA)的标记指数(LI)。ER和DR在高增殖活性组显著升高。在恶性肿瘤组中，DR和LI与病变的临床病程密切相关，在有甲状腺外或有淋巴结转移的患者中也显著升高。为了评价甲状腺癌移植瘤(AC2)中~(14)C-脱氧-D-葡萄糖(~(14&gt；C-DG))的蓄积情况，建立了甲状腺癌动物模型，通过放射自显影摄取~(14)C-DG与组织病理学检查相比较，发现~(14)C-DG的摄取与组织病理学检查结果一致。C-DG在坏死周围的肉芽组织中的含量高于在小梁生长和滤泡形成组织中存活的肿瘤细胞中的含量，提示C-DG含量的高低不仅反映了肿瘤细胞的存活和增殖，而且反映了伴随着肿瘤细胞生长的炎症和变性反应。

^<201>T1 uptake in early and delayd scan were semi-quantified as early (ER) and delayd ratio (DR).Nuclear DNA content and labeling indsx (LI) of proliferating cell nuclear antigen (PCNA) were also determined by flow cytometry (FCM) using biopsy material.ER and DR were significantly higher in the group that had high proliferative avtivity.In the group with malignant tumor, DR and LI correlated well with the clinical course of the lesions, and were also significantly higher in patients who had extra-thyroidal involvement or lymph node metastases.It is suggested that uptake of ^<201>T1, especially in delayd scan, has a potentiality to predict a proliferative activity and aggressiveness of thyroid neoplasms.An experimental model of thyroid cancer was prepared for evaluating the accumulation of ^<14>C-deoxy-D-glucose (^<14>C-DG) in thyroid cancer xenografts (AC2).By comparing autoradiographic accumulation patterns of ^<14>C-DG and histopathological examination.It was found that the uptake of ^<14>C-DG was higher in the granulation tissues surrounding necrosis than in viable tumor cells of trabeculary growing and follicle forming tissues.It is suggested that the degree of ^<14>C-DG content reflects not only tumor cell viability and proliferation but also the inflammatory and degenerative reaction accopanying tumor cell growth.