Studies on the metabolism of thyroid tumor using Nuclear Medicine

甲状腺肿瘤代谢的核医学研究

• 批准号: 04670656
• 负责人: FURUDATE Masayori
• 金额: $ 1.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670656/
• 关键词: Thyroid tumor; Nuclear Medicine; PCNA; Flow cytometry; ^<201>T1 scintigraphy; Thyroid carcinoma xenograft; ^<14>C-DG; Autoradiography,; 甲状腺腫瘍の代謝; 腫瘍増殖能; PCNAの発現率; ^<131>Iの取込み; 201Tl集積; 細胞増殖能指標; 14C-DG; 甲状腺乳頭癌自家樹立培養細胞株; ^<201>Tl集積

^<201>T1 uptake in early and delayd scan were semi-quantified as early (ER) and delayd ratio (DR).Nuclear DNA content and labeling indsx (LI) of proliferating cell nuclear antigen (PCNA) were also determined by flow cytometry (FCM) using biopsy material.ER and DR were significantly higher in the group that had high proliferative avtivity.In the group with malignant tumor, DR and LI correlated well with the clinical course of the lesions, and were also significantly higher in patients who had extra-thyroidal involvement or lymph node metastases.It is suggested that uptake of ^<201>T1, especially in delayd scan, has a potentiality to predict a proliferative activity and aggressiveness of thyroid neoplasms.An experimental model of thyroid cancer was prepared for evaluating the accumulation of ^<14>C-deoxy-D-glucose (^<14>C-DG) in thyroid cancer xenografts (AC2).By comparing autoradiographic accumulation patterns of ^<14>C-DG and histopathological examination.It was found that the uptake of ^<14>C-DG was higher in the granulation tissues surrounding necrosis than in viable tumor cells of trabeculary growing and follicle forming tissues.It is suggested that the degree of ^<14>C-DG content reflects not only tumor cell viability and proliferation but also the inflammatory and degenerative reaction accopanying tumor cell growth.

结果<201>：（1）T1摄取早期（ER）和延迟（DR）半定量，（2）增殖细胞核抗原（PCNA）标记指数（LI）和细胞核DNA含量在高增殖活性组明显增高，（3）恶性肿瘤组ER和DR明显增高，（4）恶性肿瘤组ER和DR明显增高，（5）增殖细胞核抗原（PCNA）标记指数（LI）明显增高，（6）增殖细胞核抗原（PCNA）标记指数（LI）明显增高。DR和LI与病变的临床病程密切相关，有甲状腺外受累或淋巴结转移者DR和LI明显增高，提示甲状腺癌的~（13）T_1摄取<201>，尤其是延迟扫描，本文报道了甲状腺癌移植瘤（AC 2）中~（125）<14>C-脱氧-D-葡萄糖（<14>~（125）C-DG）的蓄积情况。通过放射自显影和组织病理学检查，发现~（125）C-DG的摄取与甲状腺癌的增殖活性和侵袭性密切相关。本文还报道了~（125）C-DG在甲状腺癌移植瘤（AC 2）中的蓄积情况<14>。结果表明，~（125）C-DG的摄取与甲状腺癌的增殖活性和侵袭性密切相关。~（125）C-DG的摄取与甲状腺癌的增殖活性和侵袭性密切<14>相关DG在坏死周围肉芽组织中的含量高于小梁生长组织和滤泡形成组织中的活瘤细胞，提示~（13 C）<14>-DG含量的高低不仅反映了肿瘤细胞的活力和增殖，而且反映了肿瘤细胞生长过程中的炎症和变性反应。

项目成果

Takatoshi, Tsuda :"In vitro and vivo characterizations of established human follicular carcinoma cell line derived from thyroid cancer : A novel model for well-differentiated thyroid malignant tumor." Annals of Nuclear Medicine. 6. 159-168 (1992)

Takatoshi, Tsuda：“已建立的源自甲状腺癌的人滤泡癌细胞系的体外和体内特征：高分化甲状腺恶性肿瘤的新模型。”

Kunihiro Nakada and Masayori Furudate :"Studies on the metabolism of thyroid tumor using Nuclear Medicine." INNERVISION. 8(7). 65-66 (1993)

Kunihiro Nakada 和 Masayori Furudate：“利用核医学研究甲状腺肿瘤的代谢。”

Masahiro Kubota: "Autoradiographic analysis of ^<14>C-deoxy-D-glucose in thyroid cancer xenografts:A comparative study with pathologic correlation" Annals of Nuclear Medicine. 8(1). 75-83 (1994)

Masahiro Kubota：“甲状腺癌异种移植物中^14C-脱氧-D-葡萄糖的放射自显影分析：与病理相关性的比较研究”核医学年鉴。

中駄 邦博: "核医学的手法による甲状腺腫瘍の代謝に関する研究" INNERVISION. 8(7). 65-66 (1993)

Kunihiro Nakada：“利用核医学方法研究甲状腺肿瘤的代谢”INNERVISION 8(7) (1993)。

Takatoshi Tsuda: "In vitro and vivo characterizations of established human follicular carcinoma cell line derived from thyroid cancer:A novel model for well-differentiated thyroid malignant tumor" Annals of Nuclear Medicine. 6. 159-168 (1992)

Takatoshi Tsuda：“已建立的源自甲状腺癌的人滤泡癌细胞系的体外和体内特征：高分化甲状腺恶性肿瘤的新模型”《核医学年鉴》。