Development of Photodensitometry System Using Laser Light Source

在过去的一年里，为了将我们的激光光源光密度测量系统付诸实践，我们进行了一些实验。设备的基本设计部分改变了：PIN光电二极管和自制放大器被市面上可用的放大器取代。由于在用户界面方面具有优势的Visual Basic在MS-DOS上可用，因此由Quick Basic编程改为Visual Basic编程。针对膜扫描的条件，暂时将受光装置的狭缝直径设置为300mum，将膜的最小移动距离固定为100mum。同时也证实了其他参数可以确定为常数关系。利用该系统，可以测量薄膜上任意一点、两点之间直线上和矩形区域内的浓度。测量结果保存为文本数据。由于个人计算机主存容量（640KB）的限制和小于64KB的编程安排，因此使用一维或二维FFT和处理各种数据的其他软件，如PC-98专用的graph- software （N graph）和图像处理软件（NIH image）。期望进一步积累临床数据，包括应用于微密度测定。

During the past year, to put our photodensitometry system using a laser light source into practice, some experiments have been performed.The fundamental design of the equipment was partially changed : PIN photodiode and a homemade amplifier were replaced with commecially available ones. As Visual Basic, which is superior in its user interface, was found to be available on MS-DOS, programming by Quick Basic was changed to programming by Visual Basic. With regard to the conditions of the film scan, the diameter of the slit in the apparatus reciving light was temporarily set at 300mum, and the minimum moving distance of film was fixed at 100mum. It was also confirmed that other parameters could be determined in constant relation. With this system, the concentrations at an arbitrary point in the film, on a line between two pionts, and in a rectangular area could be measured. The results of the measurements obtained were saved as text-data.Because of limitations in the main memory volume (640KB) of the personal computer and in programming in arrangements of less than 64KB, FFT of one or two dimensions and processing of various data were used in combination with other software such as graph-softwafe (N graph) specified for the PC-98 and image processing software (NIH Image). Further accumulation of clinical data including application to microdensitometry is expected.