Cell sorting combined PCR-SSCP analysis of the p53 gene in esophageal squamous cell carcinoma

细胞分选联合PCR-SSCP分析食管鳞癌p53基因

• 批准号: 04670796
• 负责人: ISHIDA Kaoru
• 金额: $ 1.41万
• 依托单位: Iwate Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670796/
• 关键词: Tumor suppressor gene; p53; cell sorting; Rb; MCC; APC; DCC; Microsatellite; 食道癌; Microstatellite; PCR; 変異

To investigate the prognostic significance of the p53 gene abnormalities, mutation and LOH of the gene were examined by the cell sorting combined single-strand conformation polymorphism (PCR-SSCP) analysis in esophageal squamous cell carcinoma (ESCC). Further, four genetic alterations of the tumor suppressor genes (Rb, MCC,APC and DCC) were also examined by the PCR-LOH (loss of heterozygosity) assay or PCR-SSCP analysis. The cell sorting combined with PCR-SSCP analysis should improved the sensitivity of detecting p53 mutations, and provides additional information concerning the DNA ploidy pattern in endoscopic specimens of ESCCs. Although aberrations of the p53 gene were well correlated with patients' prognoses, remaining other tumor suppressor genes were not. LOH at the 3p25 detected by the microsatellite assay more freguently occurred in carcinomas with lymph node metastasis than in without it. Thus, the combination of genetic alterations examined may provide good informations in clinical use.

为探讨p53基因异常与食管鳞癌预后的关系，采用细胞分选结合单链构象多态性（PCR-SSCP）分析方法检测食管鳞癌p53基因突变和洛缺失。此外，还通过PCR-LOH（loss of heterozygosity）分析或PCR-SSCP分析检测了肿瘤抑制基因（Rb、MCC、APC和DCC）的4种遗传改变。细胞分选结合PCR-SSCP分析可提高检测p53突变的敏感性，并提供更多关于食管鳞癌内镜标本DNA倍体模式的信息。虽然p53基因的异常与患者的乳腺癌有很好的相关性，但其他肿瘤抑制基因的异常与患者的乳腺癌无关。微卫星分析发现3 p25的洛在有淋巴结转移的癌中发生率高于无淋巴结转移的癌，因此，结合基因改变的检测可为临床应用提供良好的信息。

项目成果

"Frequent microsatellite alterations on chromosome 3p in esophageal squamous cell carcinoma" Cancer Res.55. 891-894 (1995)

“食管鳞状细胞癌 3p 染色体上的频繁微卫星改变”Cancer Res.55。

Gen Tamura: "p53 mutations in esophageal cancer detected by polymerase chain reaction single‐strand conformation polymorphism analysis" Jpn. J. Cancer Res.83. 559-562 (1992)

Gen Tamura：“通过聚合酶链反应单链构象多态性分析检测食道癌中的 p53 突变”J. Cancer Res.83 (1992)。

Chihaya Maesawa: "Aberratious of tumor‐suppressor genes (p53, apc, mcc and Rb) in esophaseal squamous‐cell carcinoma" Int. J. Cancer. 57. 21-25 (1994)

Chihaya Maesawa：“食相鳞状细胞癌中肿瘤抑制基因（p53、apc、mcc 和 Rb）的异常”，《癌症杂志》57. 21-25 (1994)。

Gen Tamura et al.: "p53 mutations in esophageal cancer detected by polymerase chain reaction single-strand conformation polymorphism analysis" Jpn.J.Cancer Res.83. 559-562 (1992)

Gen Tamura 等人：“通过聚合酶链反应单链构象多态性分析检测食道癌中的 p53 突变”Jpn.J.Cancer Res.83。

Chihaya MAESAWA: "Aberrations of tumor-suppressorgenes(p53.apc.mcc and Rb)in esophogeal Sugamous-cell carcioma" International Jaeral of Cancer. 57(印刷中). 1-5 (1994)

Chihaya MAESAWA：“食管鳞状细胞癌中肿瘤抑制基因（p53.apc.mcc 和 Rb）的畸变”，International Jaeral of Cancer 57（出版中）。