Development of Bio Sensor Chip Assay

生物传感器芯片检测的开发

• 批准号: 13671332
• 负责人: ISHIDA Kaoru
• 金额: $ 2.5万
• 依托单位: Iwate Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671332/
• 关键词: Biomolecular; Interaction analysis; Bio sensor; esophageal cancer; cancer related genes; 食堂癌; 浸潤・転移; MMP; p53; syclinD1; EGFR

We developed a new system for predicting disease outcome of cancer patients, by using Bio Sensor Chip assay. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. To overcome the disadvantages of the conventional telomerase assay (TRAP : telomeric repeat amplification protocol) related to PCR artifacts and troublesome post-PCR procedures, we have developed a telomeric repeat elongation (TRE) assay which directly measures telomerase activity as the telomeric elongation rate by biosensor technology using surface plasmon resonance. The assay is rapid and sensitive method, and moreover, allows precise quantitative comparison of a wide range of human cells from somatic cells to carcinoma cells. TRE assay is suitable for practical use in the assessment of telomerase activity in preclinical and clinical trials of telomerase-based therapies, because of its reproducibility, rapidity and simplicity.

我们开发了一种新的系统，用于预测癌症患者的疾病结局，通过使用生物传感器芯片分析。该测定利用表面等离子体共振技术进行分析生物分子相互作用分析。为了克服传统的端粒酶检测（TRAP：端粒重复扩增协议）相关的PCR文物和麻烦的PCR后程序的缺点，我们已经开发了一种端粒重复延伸（TRE）检测，直接测量端粒酶活性的端粒延长率的生物传感器技术，使用表面等离子体共振。该测定法是快速和灵敏的方法，而且，允许从体细胞到癌细胞的广泛的人类细胞的精确定量比较。TRE法具有重复性好、快速、简便等优点，适用于临床前和临床试验中端粒酶活性的检测。

项目成果

Sato N, Koeda K, Ikedo K, Kimura Y., et al.: "Randomized study of the benefits of preoperative corticosteroid administration on the postoperative morbidity and cytokine response in patients undergoing surgery for esophageol cancer"Ann Surg. 236. 184-190 (

Sato N、Koeda K、Ikedo K、Kimura Y. 等人：“术前皮质类固醇给药对接受食管癌手术的患者术后发病率和细胞因子反应的益处的随机研究”Ann Surg。

Sato N, Koeda K, Ikeda K, Kimura Y, Aoki K, Iwaya T, Akiyama Y, Ishida K, Saito K, Endo S.: "Randomized study of the benefits of preoperative corticosteroid administration on the postoperative morbidity and cytokine response in patients undergoing surgery

Sato N、Koeda K、Ikeda K、Kimura Y、Aoki K、Iwaya T、Akiyama Y、Ishida K、Saito K、Endo S.：“术前皮质类固醇给药对患者术后发病率和细胞因子反应的益处的随机研究

Iijima, S, et al.: "Gastrointestinal stromal tumor of the oesophagus significance of immunohistochemical and genetic analysis of the ・・・"Eur.J Gastroenterol. 14. 445-448 (2002)

Iijima, S, 等人：“食管胃肠道间质瘤的免疫组织化学和遗传分析的意义”Eur.J Gastroenterol. 14. 445-448 (2002)

Maesawa, C., Inaba, T., Sato, H., Iijima, S., Ishida, K., Terashima, M., Sato, R., Suzuki, M., Yashima, A., Ogasawara, S., Oikawa, H., Sato, N., Saito, K., and Masuda, T.: "A rapid biosensor chip assay for measuring of telomerase activity using surface pl

前泽 C.、稻叶 T.、佐藤 H.、饭岛 S.、石田 K.、寺岛 M.、佐藤 R.、铃木 M.、屋岛 A.、小笠原 S.、

Sato N et al.: "Randomized study of the benefits of preoperative corticosteroid administration on the postoperative"Ann Surg. 236. 184-190 (2002)

Sato N 等人：“术前皮质类固醇给药对术后益处的随机研究”Ann Surg。