Immunohistochemical Study of Glutathione-related Enzyme and Proliferative Antigen in Non-Small Cell Lung Cancer

检测107例非小细胞肺癌组织中谷胱甘肽过氧化物酶(GPX)、谷胱甘肽还原酶(GR)、增殖细胞核抗原(PCNA)和表皮生长因子受体(EGFR)的表达与顺铂敏感性的关系。肿瘤细胞在顺铂溶液中孵育后，通过DNA直方图检测S期或G2M期细胞数量的增加来评价顺铂的敏感性。石蜡包埋组织用间接免疫过氧化物酶技术检测各肿瘤组织中GPX、GR、增殖细胞核抗原和表皮生长因子受体的表达。按组织学类型划分，大细胞癌、鳞癌和腺癌对顺铂的敏感率分别为40%、31%和6%。CDDP敏感性与GPX、GR表达频率呈负相关。CDDP敏感组的GPX和GR表达明显低于CDDP耐药组。而在非小细胞肺癌中，敏感组的表达明显低于敏感组，提示免疫组织化学检测这些抗原可能有助于预测非小细胞肺癌对顺铂的敏感性。

With resected tumor tissue from 107 patients with non-small cell lung cancer, the expression of glutathione peroxidase (GPX), glutathione reductase (GR), proliferating cell nuclear antigen (PCNA), and epidermal growth factor receptor (EGFR) was examined in relation to CDDP sensitivity. The CDDP sensitivity was assessed from an increase of cells in the S-phase or G2M-phase by a DNA histogram after the tumor cells were incubated in a CDDP solution. The expression of GPX,GR,PCNA and EGFR for each tumor was studied with an indirect immunoperoxidase technique on paraffin-embedded tissues.The percentage of patients sensitive to CDDP according to the histologic type was 40% for large cell carcinomas, 31% for squamous cell carcinomas, and 6% for adenocarcinomas. There was an inverse relationship between CDDP sensitivity and the frequency of GPX and GR expression. The GPX and GR expression was significantly lower in the CDDP-sensitive group than in the CDDP-resistant group. However, the expression of PCNA and EGFR was significantly lower in the sensitive group in non-small cell lung cancer.From the above findings, an immunohistochemical study of these antigens may be useful for predicting CDDP sensitivity in non-small cell lung cancer.