Glutathione Peroxidase & Redox State in Atherosclerosis

描述（由申请人提供）：活性氧（ROS）的产生和细胞氧化还原环境的变化调节细胞功能的各个方面。以前的研究氧化应激在血管疾病的重点是ROS生成系统。然而，关于血管细胞的氧化还原状态对ROS信号传导和血管疾病中细胞功能的影响知之甚少。谷胱甘肽（GSH）是细胞中最丰富的氧化还原缓冲剂。胞浆酶谷胱甘肽过氧化物酶-1（GPx-1）通过利用GSH减少过氧化氢和脂质过氧化物来保护细胞免受氧化应激。然而，氧化应激将破坏GPx-1。虽然GPx-1活性降低是冠心病患者心血管事件的预测因子，但GPx-1活性是否直接参与动脉粥样硬化的病理生理尚不清楚。该项目的中心假设是血管中GPx-1活性的变化通过NADPH氧化酶活性的改变与动脉粥样硬化的进展有因果关系。这些研究是对以往研究者对动脉粥样硬化中NADPH氧化酶衍生的ROS的观察的延伸。首先，研究将检验GPx-1活性变化对平滑肌细胞的影响是由过氧化氢诱导的氧化应激介导的假设。拟议的研究将测试的假设，细胞响应GPx-1活性的变化是依赖于细胞氧化还原的变化的持续时间。其次，研究提出，以确定GPx-1活性降低对平滑肌细胞的影响是否介导的NADPH氧化酶亚基Nox 1和Nox 4的表达。最后，研究提出了测试的假设，GPx-1活性的变化在体内影响动脉粥样硬化的进展和Nox酶的表达。从这些研究中获得的信息将为进一步研究血管疾病的氧化还原状态和潜在的新的治疗策略提供基础，以改变患者动脉粥样硬化的进展。

DESCRIPTION (provided by applicant): Production of reactive oxygen species (ROS) and changes in the cellular redox environment regulate various aspects of cellular function. Previous studies of oxidative stress in vascular disease have focused on ROS generating systems. Little is known, however, regarding effects of the redox state of vascular cells on ROS signaling and cell function in vascular disease. Glutathione (GSH) is the most abundant redox buffer in the cell. The cytosolic enzyme glutathione peroxidase-1 (GPx-1) protects the cell against oxidant stress by utilizing GSH to reduce hydrogen peroxide and lipid peroxides. Oxidative stress, however, will inactivate GPx-1. Although decreased GPx-1 activity is a predictor of cardiovascular events in patients with coronary artery disease, it is not known whether GPx-1 activity directly contributes to the pathophysiology of atherosclerosis. The central hypothesis of this project is that changes in GPx-1 activity in the blood vessel are causally related to the progression of atherosclerosis via alterations in the activity of NADPH oxidase enzymes. The proposed studies are an extension of previous observations made by the investigators of NADPH oxidase-derived ROS in atherosclerosis. First, studies will test the hypothesis that effects of changes in GPx-1 activity on smooth muscle cells are mediated by hvdroperoxide-induced oxidative stress. Proposed studies will test the hypothesis that cellular responses to changes in GPx-1 activity are dependent on the duration of the change in cellular redox. Second, studies are proposed to determine if effects of reduction in GPx-1 activity on smooth muscle cells are mediated by expression of the NADPH oxidase subunits Nox1 and Nox4. Finally, studies are proposed to test the hypothesis that changes of GPx-1 activity in vivo affect progression of atherosclerosis and the expression of Nox enzymes. Information gained from these studies will provide a foundation for additional studies of redox status in vascular disease and potential novel therapeutic strategies to modify the progression of atherosclerosis in patients.