Molecular regulation of light harvesting: Cytosolic translation control mediated by the nuclear encoded protein NAB1 in C. reinhardtii

光捕获的分子调控：莱茵衣藻核编码蛋白 NAB1 介导的胞质翻译控制

• 批准号: 44171812
• 负责人: Professor Dr. Olaf Kruse
• 金额: --
• 依托单位: Centrum für Biotechnologie (CeBiTec)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/44171812?language=en
• 关键词: Molecular; regulation; light; harvesting; Cytosolic

Translation control in cells is known as an important mechanism that regulates the expression of genes on a post-transcriptional level. In phototrophic organisms some photosynthesis genes are also controlled at the translational level. This can be of particular importance for light-regulated chloroplast proteins that are encoded in the nucleus. In this case a steady state pool of mRNA in the cytosol would enable the cell to respond faster to events in the chloroplast. However, the existence of “ready to use” mRNA requires a stress-dependent regulation system. Until recently there was only indirect evidence which indicated the existence of such post-transcriptional control. The major objective of this project is to characterise NAB1, a key protein involved in translational control of light harvesting chlorophyll binding proteins which has been identified previously by systematic screens of Chlamydomonas reinhardtii random insertion mutants. The project focuses on elucidating the mechanisms of its regulatory function and defining its role in signalling during light acclimation. Results obtained in the plant model organism Chlamydomonas will then be transferred to other organisms to evaluate if translation control is a common mechanism within the plant kingdom.

细胞中的翻译控制被认为是在转录后水平上调节基因表达的重要机制。在光养生物中，一些光合作用基因也在翻译水平上受到控制。这对于细胞核中编码的光调节叶绿体蛋白尤其重要。在这种情况下，细胞质中 mRNA 的稳态池将使细胞能够更快地响应叶绿体中的事件。然而，“即用型”mRNA 的存在需要压力依赖性调节系统。直到最近，只有间接证据表明这种转录后控制的存在。该项目的主要目标是表征 NAB1，NAB1 是一种参与光捕获叶绿素结合蛋白翻译控制的关键蛋白，之前已通过莱茵衣藻随机插入突变体的系统筛选鉴定出该蛋白。该项目的重点是阐明其调节功能的机制并确定其在光适应过程中信号传导中的作用。然后，在植物模型生物衣藻中获得的结果将被转移到其他生物体中，以评估翻译控制是否是植物界内的常见机制。

项目成果

Multi-Level Light Capture Control in Plants and Green Algae.

• DOI: 10.1016/j.tplants.2015.10.004
• 发表时间: 2016
• 期刊: Trends in plant science
• 影响因子: 20.5
• 作者: Lutz Wobbe;R. Bassi;O. Kruse

Solution structure of the RNA-binding cold-shock domain of the Chlamydomonas reinhardtii NAB1 protein and insights into RNA recognition.

• DOI: 10.1042/bj20150217
• 发表时间: 2015-07
• 期刊: The Biochemical journal
• 作者: A. Sawyer;M. Landsberg;I. Ross;O. Kruse;M. Mobli;B. Hankamer