Interactions between cel cycle regulatory genes and anti-oncogenes contributed to the malignant potential of hydatidiform mole

细胞周期调控基因和抗癌基因之间的相互作用导致葡萄胎的恶性潜力

• 批准号: 04671002
• 负责人: OHASHI Kazutomo
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671002/
• 关键词: hydatidiform mole; choriocarcinoma; chrion; oncogenes; anti-oncogenes; gene mutation

Interacition between cell cycle regulatory genes anti-oncogenes contributed to the malignant potential of hydatidiform mole.1) Expression of oncogenes and anti-oncogenes in hydatidiform mole, choriocarcinoma, and choriocarcinoma cell lines.Northern blot analyzes have revealed elevated expression of several oncogenes and decreased expression of a few anti-oncogenes in hydatidiform mole, choriocarcinoma, and choriocarcinoma cell lines. DNA deletions of a few anti-oncogenes were also detected in hydatidiform mole by southern blot analyzes. In order to detect point mutaions of oncogenes and anti-oncogenes, SSCP method was performed. cDNA of those tumors was synthesezed and used for SSCP.SSCP has revealed several point mutations in those tumors. Those mutations were confirmed by sequencing method. Some of those mutations were detected in so-called hot spot. The others were not in hot spot.2) Establishment of primaly culture system for early pregnant chorion.Chorion was separeted in the trophoblast by collagenase prepatation. Primary culture system of separeted trophoblast on collangen coated petri-dish was established.

1)葡萄胎、绒毛膜癌和绒毛膜癌细胞系中癌基因和抑癌基因的表达。Northern印迹分析显示，在葡萄胎、绒毛膜癌和绒毛膜癌细胞系中，几种癌基因的表达上调，少数抑癌基因的表达降低。Southern杂交分析还检测到葡萄胎中少数抑癌基因的DNA缺失。为了检测癌基因和抑癌基因的点突变，采用SSCP方法。合成了这些肿瘤的cDNA并用于SSCP，SSCP在这些肿瘤中发现了几个点突变。用测序的方法证实了这些突变。其中一些突变是在所谓的热点地区检测到的。2)建立早孕绒毛原代培养体系，用胶原酶预备法将绒毛膜分离到滋养层细胞。建立了分离滋养层细胞在科兰根涂层培养皿上的原代培养体系。

项目成果

Masahiko Takemura, et al.: "Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition" J.Clin. Invest.in press. (1994)

Masahiko Takemura 等人：“分娩过程中人催产素受体 mRNA 及其蛋白质在绒毛膜和蜕膜中的表达和定位”J.Clin。

Masaki Inoue, et al.: "Histogenetic analysis of ovarian germ cell tumors by DNA fingerprinting" Cancer Res. 52. 1-4 (1992)

Masaki Inoue 等人：“通过 DNA 指纹法对卵巢生殖细胞肿瘤进行组织遗传学分析”Cancer Res。

Masaki Inoue et al: "Histogenetic analysis of ovarian germ cell tumors by DNA fingerprinting" Cancer Res.52. 1-4 (1992)

Masaki Inoue 等人：“通过 DNA 指纹对卵巢生殖细胞肿瘤进行组织遗传学分析”Cancer Res.52。

Masahiko Takemura et al: "Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition" J.Clin.Invest.(in press). (1994)

Masahiko Takemura 等人：“分娩过程中人催产素受体 mRNA 及其蛋白质在绒毛膜和蜕膜中的表达和定位”J.Clin.Invest.（出版中）。

Junko Saito, et al.: "Histogenetic analysis of ovarian teratomas by DNA fingerprinting" Eur. J.Cancer. 27. 27- (1991)

Junko Saito 等人：“通过 DNA 指纹图谱对卵巢畸胎瘤进行组织遗传学分析”Eur。