The study on X-Y spirmpreseparation and Testie determining gene

对伴性遗传病患者进行配子期预选和早期胚胎选择治疗，可望治愈。本研究的主要目的是建立精子预选程序和精子X、Y的DNA探针检测方法。用荧光原位杂交（FISH）技术观察了X、Y精子的分布。建立了精子核的去凝聚程序。DNA探针穿过核膜需要用二乙苏糖醇（DTT）处理。DXY-1探针和DYZ-1探针分别作为X和Y精子的特异性DNA探针。精子性别分析的发展方向是PCR扩增法，目的是减少所需的精子细胞数。用性别决定损伤Y（SRY）基因判断精子的存在。虽然最终的目标应该是从单个精子开始分析，但它仍然是精子数量减少的过程。

The patients of sex-linked genetic deisorders have been disired to be curedby the teatment of preselection at the stage of gametes and the selection at the early embryos. Specially the objective of this study is the development of sperm preselection procedures and establishment of the detemination method for sperm X and Y using DNA probe analysisi prior to its selection. The distribution of X and Y sperm has been demonstrated by fluorescence in situ hybridization (FISH) nethod. The decondensation precedure of the sperm nuclei was established. The sperm unclei have to be treated with diethiothreitol (DTT) for getting DNA probe through nuclear membrane. DXY-1 probe and DYZ-1 have been used as specific DNA probe for X and Y sperm respectively. The development of sperm sex analysis has been directed to PCR amplification method for the purpose of reducing necessary numbers of sperm cells. The sex determinig lesion Y(SRY) gene was used to determine the existence of sperm. although final objective should be the analysisi from single sperm cell, it is still of the reducing process of sperm cell umbers.