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The effect of the enviroment of oocyte (follicular fluid, cumulus cells ) on maturation and fertilizability of oocyte.

卵母细胞的环境（卵泡液、卵丘细胞）对卵母细胞成熟和受精能力的影响。

基本信息

批准号：
60570795
负责人：
KOBAYASHI Toshifumi
金额：
$ 0.96万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

In human in vitro fertilization - embryo transfer(IVF-ET ), most of ova from small follicles under 1 ml of follicular fluid were suggested to be immature. Fertilization and cleavage of ova were relatively good from follicles of 3 or more of follicular fluid.Transient hyperprolactinemia was generally noted in women treated with hMG. In this study prolactin levels were well-correlated with serum estrogen levels. There were no differences in fertilization and cleavage rates of ova between women with higher serum prolactin levels and those with lower prolactin levels. There was even higher pregnancy rates in women with higher prolactin levels. It was, therefore, suggested that transient hyperprolactinemia seen in women at IVF-ET programs had no effect on oocyte nor follicle maturation.Cyclic AMP has inhibitory effect on oocyte maturation in mammalia. In this study, cAMP also inhibited maturation of cultured rabbit cumulusenclosed oocytes. Short term exposure of cAMP on oocyte in rabbit ovaryperfusion system even promoted the resumption of oocyte maturation.Addition of aminoglutethimide to perfusion system had no effect on germinal vesicle breakdown of rabbit ova, but significantly inhibited fertilization rates. Thus it was suggested that steroidogenesis in granulosa cells especially in cumulus cells might play an important role in cytoplasmic maturation of oocyte.

在人类体外受精-胚胎移植（IVF-ET）中，卵泡液不足1 ml的小卵泡所获卵子大多数为未成熟卵子。3个或3个以上卵泡的受精和卵裂情况相对较好，hMG治疗的妇女普遍出现一过性高泌乳素血症。在这项研究中，催乳素水平与血清雌激素水平密切相关。血清催乳素水平高的妇女与血清催乳素水平低的妇女之间卵子的受精率和卵裂率无差异。催乳素水平较高的女性怀孕率甚至更高。提示IVF-ET中出现的一过性高催乳素血症对卵母细胞和卵泡成熟无影响，而cAMP对哺乳动物卵母细胞成熟有抑制作用。cAMP对体外培养的兔卵丘卵母细胞的成熟也有抑制作用。在兔卵母细胞灌流系统中短期暴露cAMP可促进卵母细胞成熟的恢复，氨鲁米特对卵母细胞成熟泡破裂无影响，但显著抑制受精率。因此，颗粒细胞尤其是卵丘细胞的类固醇合成可能在卵母细胞胞质成熟过程中起重要作用。