Histopathelogical study of DAF on inner ear

DAF对内耳的组织病理学研究

• 批准号: 04671040
• 负责人: HARADA Tamotsu
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671040/
• 关键词: Inner ear; system of complement; DAF; stria vascularis; Corti organ; endolymphatic sac; cochlear nerve; marginal cell; 蝸牛神経; 補体系防禦機構; 血管系; 蝸中神経; 辺緑細胞

Decay-accelarating factor (DAF) is a glycoprotein in the membranes of many mammalian cells. DAF has a molecular weight of 70kDa and is inserted into membranes by a glycosylphosphatidylinositol anchor.In paroxysmal nocturnal haemoglobinuria. DAF is deficient in erythrocyte membranes, which renders them more susceptible to lysis. The purpose of this study was to determine of DAF can also be detected in inner ear. We studied the localization of DAF inner ear, using normal male guinea pigs of about 350g body weight. DAF in inner ear tissue morphology was observed as follow, 1. stria vascularis (marginal cell, intermediate cell) 2. epithelial cell in endolymphatic sac.DAF was not observed as follows, 1. organ of corti 2. Reisseners membrane 3. cochlear nerve, cochlear ganglion cell.

衰变加速因子（Decay-Accelarating Factor，简写为ERF）是一种存在于哺乳动物细胞膜上的糖蛋白。它的分子量为70 kDa，通过糖基磷脂酰肌醇锚插入细胞膜。红细胞膜中的β-内酰胺缺乏，这使得它们更容易溶解。本研究的目的是确定内耳中也可以检测到的内耳炎。本文用体重约350克的正常雄性豚鼠，对内耳的定位进行了研究。内耳组织形态学观察结果如下：1.血管纹（边缘细胞、中间细胞）2.内淋巴囊上皮细胞，未观察到淋巴结肿大。Corti器官2. Reissener膜3.耳蜗神经，耳蜗神经节细胞。

项目成果

T.Harada: "Mesurement of Anaphylatoxin Activity durin surgery" Acta Otolaryngol. 501. 88-91 (1993)

T.Harada：“手术期间过敏毒素活性的测量”Acta Otolaryngol。

T.Harada: "The effect of Anaphylatoxin Component on Inner ear Damage" Acta Otolaryngol. 112. 265-271 (1992)

T.Harada：“过敏毒素成分对内耳损伤的影响”Acta Otolaryngol。

原田 保: "腎不全患者の内耳疾患と免疫複合体の関連について" Equilibrium Res. 9. 55-59 (1993)

Tamotsu Harada：“肾衰竭患者内耳疾病与免疫复合物的关系”平衡研究 (Equilibrium Res)。 9. 55-59 (1993)

K.Hong, T.Harada: "Binding ability of complement receptor CRI to C3 bound on the surface of M^+ group A streptococci" Immunology. 80. 640-644 (1993)

K.Hong、T.Harada：“补体受体 CRI 与 M 组 A 链球菌表面结合的 C3 的结合能力”免疫学。

T.Harada: "The mechanism of immune complex-mediated inner ear diseases." Ann Otol Rhinol Laryngol. 101. 72-77 (1992)

T.Harada：“免疫复合物介导的内耳疾病的机制。”