The histologic and functional study of macrophage in the endolymphatic sac.

内淋巴囊巨噬细胞的组织学和功能研究。

• 批准号: 11671715
• 负责人: HARADA Tamotsu
• 金额: $ 1.28万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671715/
• 关键词: endolymphatic sac; macrophage; endolymphatic hydrops; Cytokine; electrocochleogram; アナフィラトキシン

Opinions have been divided on the cause of Meniere's disease. It's pathology was first described by Yamakawa (1938) who found endolymphatic hydrops. He have reported the degeneration of the endolymphatic sac. The purpose of this study was to elucidate macrophage functions in the endolymphatic sac.1.Anaphylatoxins were inoculated into the carotid artery of the guinea pigs (n=13). Pathological changes in inner ears were observed by light microscopy and counted macrophages in the whole endolymphatic sac. The stria vascularis were extremely atrophied. Macrophages were found 77+ 40 pieces (P) in the endolymphatic sac (control : 22ア 13P). Macrophages were found 2.3P in the endolymphatic sac of CH3/He J mouse, 4.6P in the endolymphatic sac of CH/He mouse.2.We could measure electrocochleogram on mice.3.Macrophages in endolymphatic sac were cultured in Dulbecco' s - MEM supplemented with 10% fetal bovine serum. We measured the inflammatory substances (IL- /3, TNF- a, IL-4, IL-6) levels in DulbeccoVMEM, but we couldn't detect the inflammatory substances.The inflammatory function of macrophages in the endolymphatic sac may play an important role but we surmised these macrophages were directly unrelated to the endolymphatic hydrops.

关于梅尼埃病的病因一直存在分歧。它的病理学首先由Yamakawa（1938）描述，他发现了内淋巴积水。他报告了内淋巴囊的退化。本研究的目的是阐明内淋巴囊内巨噬细胞的功能。1.将过敏毒素接种于豚鼠（n=13）颈动脉。光镜下观察内耳病理改变，计数整个内淋巴囊内巨噬细胞。血管纹严重萎缩。内淋巴囊内巨噬细胞77 ± 40个（P <0.01），对照组为22 ± 13个（P <0.01）。结果表明：CH_3/He_J小鼠内淋巴囊内有2.3P的巨噬细胞，CH/He小鼠内淋巴囊内有4.6P的巨噬细胞;我们检测了DulbeccoVMEM培养液中的炎性物质（IL-3、TNF-α、IL-4、IL-6），但未检测到炎性物质，推测内淋巴囊内巨噬细胞的炎性功能可能起重要作用，但推测这些巨噬细胞与内淋巴积水无直接关系。

项目成果

原田保: "耳鼻咽喉科診療プラクティス"文光堂. 259 (2001)

原田保：《耳鼻喉科实践》文科堂 259 (2001)。

原田 保: "いろいろな難聴と人工内耳の適応について"ろう教育科学. 42・1. 1-4 (2000)

原田保：“关于各种类型的听力损失和人工耳蜗的适应”《聋人教育科学》42・1（2000）。

TAMOTSU HARADA: "Various Hard of Hearing and Application of Cochlear Implant."SCIENCE OF EDUCATIONAL DEAFNESS. 42(1). 1-4 (2000)

TAMOTSU HARADA：“各种听力障碍与人工耳蜗的应用。”教育性耳聋科学。

原田 保: "内耳疾患と免疫"耳鼻臨床. 94・11. 957-962 (2001)

原田保：“内耳疾病和免疫”耳鼻喉科94・11（2001）。

原田 保(分担): "耳鼻咽喉科診療プラクテイス"文光堂. 259 (2001)

原田保（撰稿人）：《耳鼻喉科实践》文科堂 259 (2001)。