Observations of intracellular Ca^<2+> and Cl-concentrations in inner ear isolated cells

内耳离体细胞内Ca^2和Cl浓度的观察

• 批准号: 04671056
• 负责人: YAMASHITA Toshio
• 金额: $ 1.28万
• 依托单位: KANSAI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671056/
• 关键词: Cochlear hair cells; vestibular hair cells; spiral ganglion cells; intracellular Ca^<2+> concentration; intracellular Cl- concentration16FA06 : fura-2; 画像解析法; 蝸牛有毛細胞; 細胞内Ca^<2+>濃度; Ach; ATP; gultamate; 前庭器有毛細胞; 蝸牛内有毛細胞; 遠心性神経伝達物質; 細胞内遊離Ca^<2

1) To elucidate efferent inhibitory mechanisms of the cochlear hair cells and vestibular hair cells, the effects of candidate efferent neurotransmitters on intracellular Ca^<2+> concentrations ([Ca^<2+>] i) in these isolated cells of guinea pig were determined. Both Ach and ATP seem to be efferent neurotransmitters, however, CGRP and Enk were thought to be functioning as efferent neuromodulators.2) Slow shortening of cochlear outer hair cells has been speculated to modify cochlear sensitivity. Tetanic electrical field stimulation of isolated outer hair cells from guinea pigs shortened the cells for 2-3 min. Electrical stimulation reduced cell length and volume and decreased the intracellular Cl-concentration. These findings suggest that these shortening and shrinkage result from Cl- efflux.3) [Ca^<2+>] i is isolated spiral ganglion cells (SGCs) of the guinea pig cochlear were measured using the Ca^<2+> sensitive fluorescence dye fura-2. L-gultamate increased [Ca^<2+>] i in SGCs with neuritic processes. We thus propose that the excitatory amino acid, probably L-gultamate, may function as a neurotransmitter of the hair cell-afferent nerve synapse in the guinea pig cochlea.

1)为了阐明耳蜗毛细胞和前庭毛细胞的传出抑制机制，确定了候选传出神经递质对豚鼠这些分离细胞中细胞内Ca^2+浓度([Ca^2+]i)的影响。 Ach 和 ATP 似乎都是传出神经递质，然而，CGRP 和 Enk 被认为起着传出神经调节剂的作用。2) 据推测，耳蜗外毛细胞的缓慢缩短可以改变耳蜗的敏感性。对豚鼠分离的外毛细胞进行强直电场刺激，可使细胞缩短 2-3 分钟。电刺激减少了细胞长度和体积，并降低了细胞内 Cl 浓度。这些发现表明这些缩短和收缩是由Cl-流出引起的。3)[Ca 2+ ] i 是使用Ca 2+ 敏感荧光染料fura-2测量豚鼠耳蜗的分离螺旋神经节细胞(SGC)。 L-谷氨酸增加具有神经炎过程的SGC中的[Ca ^ 2+ ] i 。因此，我们提出兴奋性氨基酸（可能是 L-谷氨酸）可能充当豚鼠耳蜗毛细胞传入神经突触的神经递质。

项目成果

韓東一: "Calcium mobilization in isolated cochlear spiral ganglion cells of the guinea pig" Acta Otolaryngol(Stockh). Suppl.506. 26-29 (1993)

Dong-il Han：“豚鼠离体耳蜗螺旋神经节细胞中的钙动员”Acta Otolaryngol (Stockh)。 26-29 (1993)。

Ohnishi S,Yamashita T,et al.: "Regulation of Cl^- conductance in delayed shortening and Shrinkage of outer hair cells" Acta Otolaryngol (Stockh) Suppl.500. 42-45 (1993)

Ohnishi S、Yamashita T 等人：“外毛细胞延迟缩短和收缩中 Cl^- 电导的调节”Acta Otolaryngol (Stockh) Suppl.500。

大谷真喜子: "Thermal influence on intercellular calcium concentration in vestibular hair cells isolated from the guinea pig" Acta Otolaryngol(Stockh). Suppl.500. 46-49 (1993)

Makiko Otani：“热对豚鼠前庭毛细胞中钙浓度的影响”Acta Otolaryngol (Stockh) 46-49 (1993)。

Yamashita T,et al.: "Effects of efferent neurotransmitters on intracellular Ca^<2+> concentration in vestibular hair cells of the quinea pig" Acta Otolaryngol (Stockh) Suppl.500. 26-30 (1993)

Yamashita T，et al.：“传出神经递质对奎尼亚猪前庭毛细胞内Ca 2+ 浓度的影响”Acta Otolaryngol (Stockh) Suppl.500。

T.Yamashita,H.Amano,T.Kumazawa: "Efferent Neurotransmitters and Intracellular Ca^<2+> Concentrations in Inner Hair Cells of Guinea Pig" ORL. 55. 201-204 (1993)

T.Yamashita、H.Amano、T.Kumazawa：“豚鼠内毛细胞中的传出神经递质和细胞内 Ca^<2> 浓度”ORL。