Ocular hypotensive effect and intra-cellular signal transduction system of prostaglardins.

前列腺素的降眼压作用及细胞内信号转导系统。

• 批准号: 04671075
• 负责人: MISHIMA Hiromu
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671075/
• 关键词: Prostaglandins; Intra-cellular signaling system; Inositol triphospholipid (IP_3); Cyclic AMP (_cAMP); Ciliary epithelia; Blood aqueous barrier; Intraocular pressure; イノシトール1,4,5・三燐酸(IP_3)

Resently, prostaglandins (PG_s) have been identified as a new bio-active material. We attempted to clarify the relationships between PG_s and intracellular signaling systems, and the ocular hypotensive effect of PGF_<2alpha>. Then we investigated the effects of PG_s on blood aqueous barrier (BAB) and ciliary epithelia.1. PGF_<2alpha> activates the inositol phospholipid turnover system, while PGD_2 and PGE_2 activate the _cAMP system.2. Topical administration of phXA41, a prostaglandin F_<2alpha>-isopropyl ester analogue, caused marked intraocular pressure reduction in a dose-dependent manner in primary open-angle glaucoma patients. After long term apprication of phXA41 caused no aqueous flare rise, which means no effect on BAB.3. There was no morphological changes detected in the iridial portion of ciliary processes in rabit's eye.

近年来，洋地黄素（PG_s）被发现是一种新的生物活性物质。本研究试图阐明PG_s与细胞内信号系统的关系，以及PGF_s的眼扩张作用<2alpha>。然后观察PG_s对血-房水屏障（blood aqueous barrier，BAB）和睫状体上皮的影响. PGF_2<2alpha>激活肌醇磷脂周转系统，而PGD_2和PGE_2激活cAMP系统.局部应用前列腺素F_<2alpha>-异丙酯类似物phXA_（41）可使原发性开角型青光眼患者的眼压明显降低，并呈剂量依赖性。长期应用phXA 41后，未引起房水潮红升高，说明对BAB无影响。兔眼睫状突虹膜部未见形态学改变。

项目成果

小坂敏哉: "各種プロスタグランジン長期間点眼後の家兎毛様体の形態学的変化-毛様体突起虹彩部の変化-." 広島大学医学雑誌. 42(印刷中). (1994)

Toshiya Kosaka：“长期滴注各种前列腺素后家兔睫状体的形态变化 - 睫状突和虹膜区域的变化。” 42（出版中）。

小坂敏哉: "各種プロスタグランジン長期間点眼後の家兎毛様体の形態学的変化-毛様体突起虹彩部の変化-" 広島大学医学雑誌. 42(印刷中). (1994)

Toshiya Kosaka：“长期滴注各种前列腺素后家兔睫状体的形态变化 - 睫状突和虹膜区域的变化 -”广岛大学医学杂志 42（出版中）。

Hotehema Y: "Clinical efficacy of phXA34 and phXA41, two novel prostaglandin F_<2alpha>-isopropyl ester analogues for glaucoma treatment." Jpn J Ophthalmol. 37. 256-269 (1993)

Hothema Y：“phXA34 和 phXA41 这两种新型前列腺素 F_2α-异丙酯类似物治疗青光眼的临床疗效。”

Kosaka T: "The effects of topical application prostaglandins on the rabbit iridial portion of ciliary process : light and electron microscope sudies after the long-term application." Hiroshima M.J.42 (in press.). (1994)

Kosaka T：“局部应用前列腺素对兔睫状突虹膜部分的影响：长期应用后的光学和电子显微镜研究。”

Hotehema Y: "Clinical efficacy of PhXA34 and PhXA41,two novel prostaglandin F_<2α>-isopropyl ester analogues for glaucoma treatment." Jpn J Ophthalmol. 37. 256-269 (1993)

Hothema Y：“PhXA34 和 PhXA41，两种新型前列腺素 F_2α-异丙酯类似物治疗青光眼的临床疗效。” Jpn J Ophamol. 37. 256-269 (1993)