Roll of cellular Signalling systems on intraocular pressure regulation mechanisms.

细胞信号系统对眼压调节机制的影响。

• 批准号: 02670788
• 负责人: MISHIMA Hiromu
• 金额: $ 1.6万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670788/
• 关键词: Adenosine 3' : 5'-cyclic monophosphate (cAMP); Protein kinase C (PKC); Inositol phospholipid turnover system; Cross-talk between cellular signalling systems; Cultured ciliary epithelia (CE); プロテインキナ-ゼ C; プロテインキナ-ゼC(PKC); イノシト-ル1、4、5ー三燐酸(IP3); 毛様体上

We studied how drugs that alter the inositol phospholopid turnover system affected the adenosine 3' : 5'-cyclic monophosphate(cAMP)system in ciliary epithelial cells to clarify a cross-talk between cAMP system and inositol phospholipid turnover system.The cAMP increases were observed in cultured chick embryo ciliary epithelial cells stimulated with isoproterenol, forskolin or NaF. The cultured cells pretreated with 2OOnM phorbol 12myristate 13-acetate(PMA), a protein kinase C(PKC)activator showed 42% inhibition of the cAMP increase induced by isoproterenol. When cultured cells incubated with 5OuM H-7, a PKC inhibitor were pretreated with 2OOnti PMA and stimulated with isoproterenol, the inhibitory effect on cAMP increase was reduced.The cultured cells pretreated with 2OOnM PMA showed 12% inhibition of the cAMP increase induced by forskolin. No inhibition of the cAMP increase was noted in the cultured cells stimulated with NaF after 2OOnM PMA pretreatment.These findings suggest that inhibition of the cAMP system is caused by the activation of PKC. -adrenergic receptor was regarded as the affected point of PKC.

我们研究了改变肌醇磷脂转换系统的药物如何影响纤毛上皮细胞中腺苷3':5'-环单磷酸腺苷（cAMP）系统，以阐明cAMP系统与肌醇磷脂转换系统之间的交叉对话。异丙肾上腺素、福斯克林和NaF刺激培养的鸡胚纤毛上皮细胞cAMP升高。蛋白激酶C（PKC）激活剂PMA预处理后的培养细胞对异丙肾上腺素诱导的cAMP升高有42%的抑制作用。用PKC抑制剂5OuM H-7孵育培养的细胞，用20onti PMA预处理，用异丙肾上腺素刺激，对cAMP增加的抑制作用减弱。经20onm PMA预处理的培养细胞对福斯克林诱导的cAMP增加有12%的抑制作用。经2onm PMA预处理后，NaF刺激的培养细胞中cAMP的增加未见抑制作用。这些发现表明cAMP系统的抑制是由PKC的激活引起的。-肾上腺素能受体是PKC的影响点。