Comparison between mRNA expression by RT-PCR method in human gingiva of periodontitis and clinical finding

牙周炎人牙龈组织RT-PCR法mRNA表达比较及临床发现

• 批准号: 04671101
• 负责人: TAKAHASHI Hiroshi
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671101/
• 关键词: Gingiva; Periodontitis; RT-PCR method; mRNA; Cytokine; 細胞接着分子; 受容体系遺伝子

The mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) method and immunohistochemistry of cytokine were studied with gingival biopsies from 20 cases of adult periodontitis and 4 cases of rapidly progressive periodontitis. Twenty-four cases were divided into two groups of mild type and severe type, which were defined by depth of periodontal pocket. The depth of periodontal pocket which is less than 6 mm is the feature of mild periodontitis. The severe periodontitis is characterized by the increased depth of periodontal pocket (more than 6 mm).Immunohistochemical study showed that immunocompetent cells in mild periodontitis reacted with interleukin 2,3 and 4. By contrast, interleukin 1alpha, 1beta, 2, 4 and 6 positive cells were frequently observed in severe periodontitis. By RT-PCR method, mRNA expression of interleukin 1beta in severe periodontitis was significantly higher than that of interleukin 1alpha in severe periodontitis.Expression of mRNA of interleukin 2, 3, 4, 5 and 6 was not detected in the inflamed gingiva.These results suggest that interleukin 1beta may play the important role of pathogenesis and chronic status.

应用逆转录-聚合酶链反应（RT-PCR）和免疫组化方法对20例成人牙周炎和4例快速进展性牙周炎牙龈组织中细胞因子mRNA的表达进行了研究。根据牙周袋深度将24例患者分为轻度和重度两组。牙周袋深度小于6 mm是轻度牙周炎的特征。免疫组化结果显示，轻度牙周炎牙周组织中免疫活性细胞与白细胞介素2、3、4呈阳性反应。而IL-1 α、IL-1 β、IL-2、IL-4和IL-6阳性细胞在重度牙周炎中多见。RT-PCR检测IL-1 β mRNA在重度牙周炎中的表达明显高于IL-1 α，而IL-2、3、4、5、6在炎症牙龈中均未检测到mRNA表达，提示IL-1 β可能在牙周炎的发病机制和慢性状态中起重要作用。

项目成果

Pe MB: "Ultrastructural demonstration of rat myofibroblasts in phenytoin-induced gingival overgrowth." Japan Journal of Oral Biology. 35. 496-504 (1993)

Pe MB：“苯妥英诱导的牙龈过度生长中大鼠肌成纤维细胞的超微结构演示。”

Kenji Imura, Yoshitaka Hara, Ihachi Kato: "A Histological Study on Participation of the Immune System in gingival Inflammation and Osteoclastic Formation Induced by Endotoxin Derived from Escherichia coli." Journal of the Japanese Society of Periodontolog

Kenji Imura、Yoshitaka Hara、Ihachi Kato：“免疫系统参与大肠杆菌内毒素诱导的牙龈炎症和破骨细胞形成的组织学研究”。

Hiroshi Takahashi, Shuichi Fujita, Haruo Okabe, Nobuo Tsuda, Fumiaki Tezuka: "Estimation of Silver-Binding Nucleolar Organizer Regions (AgNORs) in Lymphoproliferative Disorders of Gastrointestinal Tract." Pathology, Research and Practice. (in press). (199

Hiroshi Takahashi、Shuichi Fujita、Haruo Okabe、Nobuo Tsuda、Fumiaki Tezuka：“胃肠道淋巴增殖性疾病中银结合核仁组织区域 (AgNOR) 的估计”。

Shuichi Fujita: "Nucleolar organizer regions in malignant salivary gland tumors." Acta Pathologica Japonica. 42. 483-486 (1992)

Shuichi Fujita：“恶性唾液腺肿瘤中的核仁组织区域。”

Hiroshi Takahashi, Shuichi Fujita, Jun Cheng, Nobuo Tsuda, Fumiaki Tezuka, Ai-Ru Liu, Haruo Okabe: "Nucleolar organizer regions (NORs) in lymphoproliferative disorders of the human salivary gland." Analytical Cellular Pathology. 6. 51-63 (1994)

Hiroshi Takahashi、Shuichi Fujita、Jun Cheng、Nobuo Tsuda、Fumiaki Tezuka、Ai-Ru Liu、Haruo Okabe：“人类唾液腺淋巴增殖性疾病中的核仁组织区 (NOR)。”