Detection of HBV and HCV by PCR in Saliva

PCR 法检测唾液中的 HBV 和 HCV

• 批准号: 04671109
• 负责人: KOMIYAMA Kazuo
• 金额: $ 1.28万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671109/
• 关键词: HBV; HCV; PCR; Southern hybridization; Saliva; RT-PCR; B型肝炎ウイルス; C型肝炎ウイルス; 唾液

Both of HBV and HCV are major cosative pathogen for hepatitis. It is well known that these virus were often transmitted by blood. Human saliva occasionally contains various viral agents including hepatitis virus, suggesting a possible source of hepatitis virus infection through saliva. In addition, dental practice led to bleeding during tooth extraction or treatment for periodontitis. During treatment or after remission of hepatitis patients sometimes visit dental clinics for the treatment of oral disease. We have examined the presence of HBV and HCV by PCR in the saliva of patients with various liver condition including chronic hepatitis. Occult blood contained saliva of these patients(75%). HBV was detected in high ratio among healthy carrier(75%), not only for chronic hepatitis patients(42%). HCV was often deteced in chronic hepatitis(35%), liver chirrhosis(73%) and hepatoma(35%) patient. HCV also positive for saliva from dental patient(50%). It is thus clear that HBV and HCV have been detected in the saliva of patient with various liver condition who under treatment for oral disease and dentist shoud aware of the possibility of HBV and HCV infection via saliva contactd during oral treatment.

HBV和HCV都是肝炎的主要肌肉病原体。众所周知，这些病毒通常是由血液传播的。人类唾液有时会含有各种病毒剂，包括肝炎病毒，这表明通过唾液感染了丙型肝炎病毒。此外，牙科实践导致牙齿拔牙或牙周炎治疗期间出血。在治疗期间或缓解肝炎患者后，有时会访问牙科诊所以治疗口腔疾病。我们已经在患有各种肝病（包括慢性肝炎）患者的唾液中检查了PCR的HBV和HCV的存在。神秘血液中含有这些患者的唾液（75％）。在健康载体（75％）中，不仅针对慢性肝炎患者（42％）检测到HBV的比例很高（75％）。 HCV经常在慢性肝炎（35％），肝chirhosis（73％）和肝癌（35％）患者中进行检测。牙科患者的唾液（50％）的HCV也呈阳性。因此，很明显，在患有各种肝病的患者的唾液中发现了HBV和HCV，他们正在接受口腔疾病治疗，并且牙医知道在口腔治疗期间通过唾液接触的HBV和HCV感染的可能性。

项目成果

Yumiko Matuda: "Detection of HBV-DNA by Polymerase Chain Reaction" Nihon University Dental Journal. 66. 638-649 (1992)

Yumiko Matuda：“通过聚合酶链反应检测 HBV-DNA”日本大学牙科杂志。

小宮山一雄: "肝炎ウイルス研究の現況-C型肝炎を中心に-" 東京歯科医師会誌. 41. 245-252 (1993)

Kazuo Komiyama：“肝炎病毒研究的现状 - 关注丙型肝炎”东京牙科协会杂志 41. 245-252 (1993)。

松田由紀子: "Polymerase chain Reaction法を用いたB型肝炎ウイルスの検出" 日大歯学. 66. 638-649 (1992)

Yukiko Matsuda：“使用聚合酶链反应法检测乙型肝炎病毒”日本大学牙科 66. 638-649 (1992)。

小宮山 一雄: "肝炎ウイルス研究の現況-C型肝炎を中心に-" 東京都歯科医師会雑誌. 41. 245-252 (1993)

Kazuo Komiyama：“肝炎病毒研究的现状 - 关注丙型肝炎”东京牙科协会杂志 41. 245-252 (1993)。

Kazuo Komiyama: "HCV research up to date" The Journal of the Tokyo Dental Association. 41. 245-252 (1993)

小宫山一夫：“HCV 研究最新进展”，东京牙科协会杂志。