Detection of HBV and HCV by PCR in Saliva

乙肝病毒和丙型肝炎病毒都是肝炎的主要致病原。众所周知，这些病毒经常通过血液传播。人类唾液中偶尔含有包括肝炎病毒在内的各种病毒病原体，这表明肝炎病毒可能是通过唾液感染的。此外，在拔牙或牙周炎治疗过程中，牙科实践会导致出血。肝炎患者在治疗期间或缓解后，有时会去牙科诊所治疗口腔疾病。我们用聚合酶链式反应检测了包括慢性肝炎在内的各种肝病患者唾液中乙肝病毒和丙型肝炎病毒的存在。隐血中含有唾液(75%)。乙肝病毒在健康携带者中的检出比例很高(75%)，而不仅仅是慢性肝炎患者(42%)。慢性肝炎(35%)、肝硬变(73%)、肝癌(35%)患者常检出丙型肝炎病毒。牙科患者唾液中丙型肝炎病毒也呈阳性(50%)。由此可见，在接受口腔疾病治疗的各种肝病患者的唾液中均检出了乙肝病毒和丙型肝炎病毒，牙科医生在口腔治疗过程中应注意唾液接触感染的可能性。

Both of HBV and HCV are major cosative pathogen for hepatitis. It is well known that these virus were often transmitted by blood. Human saliva occasionally contains various viral agents including hepatitis virus, suggesting a possible source of hepatitis virus infection through saliva. In addition, dental practice led to bleeding during tooth extraction or treatment for periodontitis. During treatment or after remission of hepatitis patients sometimes visit dental clinics for the treatment of oral disease. We have examined the presence of HBV and HCV by PCR in the saliva of patients with various liver condition including chronic hepatitis. Occult blood contained saliva of these patients(75%). HBV was detected in high ratio among healthy carrier(75%), not only for chronic hepatitis patients(42%). HCV was often deteced in chronic hepatitis(35%), liver chirrhosis(73%) and hepatoma(35%) patient. HCV also positive for saliva from dental patient(50%). It is thus clear that HBV and HCV have been detected in the saliva of patient with various liver condition who under treatment for oral disease and dentist shoud aware of the possibility of HBV and HCV infection via saliva contactd during oral treatment.