Elucidation of the disease molecules in oral lichen planus using proteome analysis.

为了阐明口腔粘膜难治性病变的疾病状态，如口腔扁平苔藓，我们采用了LC-MS/MS分析。共检测到92个口腔扁平苔藓特异性蛋白。并与上皮异常增生、原位癌、口腔粘膜癌进行比较。我们发现细胞角蛋白在口腔扁平苔藓和口腔原位癌中表达减少，而在口腔浸润性癌中表达消失。然而，CK 17的表达增加的过程中的上皮异型增生和膀胱炎的进展。CK的这种矛盾表达模式有助于这些恶性交界性病变的鉴别诊断。

To clarify the disease status of an intractable lesion of oral mucosa, such as oral lichen planus, we have employed the LC-MS/MS analysis. 92 proteins of specific for the oral lichen planus have detected. The data compared with epithelial dysplasia, carcinoma in situ and carcinoma of oral mucosa. We have found different expression pattern of cytokeratins, which is decreased CK13 expression in the oral lichen planus and carcinoma in situ as well as disappearance in the invasive carcinoma. However, CK17 expression increased in a course of progression of the epithelial dysplasia and atypia. This contradict expression pattern of CK is usefulness for the differential diagnosis in these malignant borderline lesions.