Study on the interleukin-2 producing capacity in the patients with periodontitis

牙周炎患者白细胞介素2产生能力的研究

• 批准号: 04671159
• 负责人: ARAI Hideo
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671159/
• 关键词: Periodontitis; Interleukin-2; Intracellular signal transduction; T cell; CD3; Interleukin-2(IL-2); IL-2レセプター; 若年性歯周炎; 細胞内Ca^<2+>濃度; phorbol myristate acetate(PMA); ionomycine; 細胞内情報伝達系

The purpose of this study was to assess the role of interleukin-2 (IL-2) in the pathogenesis of periodontitis. The levels of IL-2 produced from mononuclear cells were measured after stimulating with a CD3 monoclonal antibody (CD3 mAb) in 45 patients (12 with juvenile periodontitis [JP], 20 with rapidly progressive periodontitis and 13 with adult periodontitis) and 20 healthy subjects (HS). Six subjects including 3 JP patients demonstrated elevated IL-2 level. When phorbol myristate (PMA) was substituted for monocytes, there existed one JP patient with significantly high IL-2 producing capacity, and two JP patients and one HS with low capacity. To investigate the cause of their unusual IL-2 producing capacities, intracellular signal transduction system in T cells was examined. The levels of IL-2 produced upon stimulation with ionomycin and PMA were proportionate to those produced upon stimulation with CD3 mAb and PMA in the subjects examined except one JP patient ; suggesting that the elevated IL-2 producing capacity of this patient is related to the intracellular calcium ion level after stimulation of CD3 molecules. These results suggest that IL-2 producing capacity is responsible for the pathogenesis of a certain type of early-onset periodontitis, and that intracellular signal transduction system is concerned with the imbalance of IL-2 production in some individuals.

本研究旨在探讨白细胞介素2（IL-2）在牙周炎发病机制中的作用。用CD 3单克隆抗体（CD 3 mAb）刺激45例牙周炎患者（12例青少年牙周炎，20例快速进展性牙周炎，13例成人牙周炎）和20例健康人（HS）的单个核细胞，测定其产生IL-2的水平。6例受试者（包括3例JP患者）显示IL-2水平升高。当用佛波醇肉豆蔻酸酯（PMA）替代单核细胞时，有1例JP患者具有显著高的IL-2产生能力，而2例JP患者和1例HS具有低的IL-2产生能力。为了研究其异常IL-2产生能力的原因，检测了T细胞中的细胞内信号转导系统。除1例JP患者外，在受试者中，离子霉素和PMA刺激后产生的IL-2水平与CD 3 mAb和PMA刺激后产生的IL-2水平成比例;这表明该患者IL-2产生能力升高与CD 3分子刺激后的细胞内钙离子水平相关。提示IL-2产生能力与某些早发性牙周炎的发病机制有关，细胞内信号转导系统与某些个体IL-2产生失衡有关。