Study on the control of periodontal ligament fibroblast functions by transforming growth factor

转化生长因子调控牙周膜成纤维细胞功能的研究

• 批准号: 08457506
• 负责人: ARAI Hideo
• 金额: $ 3.07万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457506/
• 关键词: Periodontal ligament fibroblast; Transforming growth factor-beta (TGF-beta); TGF-beta receptor; Periodontal tissue regeneration; Regulation of cell function; TGF-β; セリンスレオニンキナーゼレセプター発現; 機械的外力; in situハイブリダイゼーション; トランスフォーミング成長因子(TGF-β); DNA合成; コ

TGF-beta has been shown to play a critical role in the stimulation and regulation of the wound healing process. Some studies focused on the characteristic of TGF-beta as a biological mediators in periodontal tissue. The purpose of this study is to understand the role of TGF-beta in periodontal tissue regeneration. We examined the effect of TGF-beta on the cellular functions of human periodontal ligament fibroblasts (HPLF) and the kinetics of the expression of TGF-beta and their receptors in HPLF.We got the following results.1. TGF-beta enhanced DNA synthesis, collagen synthesis, non-collagen synthesis and alkaline phosphatase (ALP) activity as a bone formation marker in HPLF in vitro.2. HPLF expressed TGF-beta genes and TGF-beta receptor genes (type I, II, III) in vitro3. The expression of TGF-beta genes and TGF-beta receptor genes decreased in response to assumptive mechanical stress such as a occlusal force in cultured HPLF.In vivo TGF-beta genes were detected in periodontal ligament cells of rat periodontal tissue by using in situ hybridization. The amount of TGF-beta gene expression in periodontal ligament cells is larger than gingival fibroblasts.These results suggest that TGF-beta plays important role in regulation several key cellular functions of HPLF such as proliferation, cell matrix synthesis, and metabolism of hard tissue in periodontal tissue regeneration and that the mechanisms are regulated in autocrine system and in response to mechanical stress.

TGF-β已被证明在刺激和调节伤口愈合过程中起关键作用。近年来，TGF-β作为牙周组织中的生物介质的特性受到了广泛关注。本研究的目的是了解TGF-β在牙周组织再生中的作用。本研究通过观察TGF-β对人牙周膜成纤维细胞（HPLF）细胞功能的影响，以及TGF-β及其受体在HPLF中表达的动态变化，获得以下结果. TGF-β在体外可促进HPLF的DNA合成、胶原合成、非胶原合成和碱性磷酸酶（ALP）活性，可作为成骨标志物. HPLF在体外表达TGF-β基因和TGF-β受体基因（I、II、III型）3。TGF-β基因和TGF-β受体基因的表达随着咬合力等机械应力的作用而降低。采用原位杂交技术检测大鼠牙周膜细胞TGF-β基因的表达。TGF-β基因在牙周膜细胞中的表达量高于牙龈成纤维细胞，提示TGF-β在牙周组织再生过程中对HPLF的增殖、细胞基质合成、硬组织代谢等关键细胞功能起重要调节作用，其调节机制可能是通过自分泌系统和机械应力反应实现的。

项目成果

Arai, H., Nishimura, F., Washio, N., Takashiba, S.et al.: "The inhibition of DNA synthesis by prostaglandin E_2 in human gingival fibroblasts is independent of the cycle AMP-protein kinase A signal transduction pathway" J.Periodont.Res.33. 33-39 (1998)

Arai, H.、Nishimura, F.、Washio, N.、Takashiba, S.等人：“人牙龈成纤维细胞中前列腺素 E_2 对 DNA 合成的抑制与循环 AMP-蛋白激酶 A 信号转导途径无关”

Nishimura, F., Takashiba, S., et al.: "Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors" Oral Diseases. 3. 162-166 (1997)

Nishimura, F.、Takashiba, S. 等人：“青少年和老年供体中人牙周膜细胞体外增殖能力的比较”口腔疾病。

H.H.Chou,S.Takashiba,M.Takigawa,F.Nishimura,H.Arai,Y.Murayama: "Role of Type II Interfeukin-1 Receptor Expressed on Human Gingiral Fibroblasts." Journal of Dental Research. Volume 76 Special Issue. 406- (1997)

H.H.Chou、S.Takashiba、M.Takikawa、F.Nishimura、H.Arai、Y.Murayama：“II 型 Interfeukin-1 受体在人牙龈成纤维细胞上表达的作用。”

樋口 豊, 滝川雅之, 新井英雄, 鷲尾憲文, 高柴正悟ら: "ヒト歯肉線維芽細胞のDNA合成および細胞外基質合成に及ぼすTNF-αの影響" 日本歯周病学会会誌. 39. 264-272 (1997)

Yutaka Higuchi、Masayuki Takikawa、Hideo Arai、Norifumi Washio、Masago Takashiba 等人：“TNF-α 对人牙龈成纤维细胞 DNA 合成和细胞外基质合成的影响”日本牙周病学会杂志 39. 264-272。 (1997)

Kono, T., Takigawa, M., Takashiba, S., Arai, H.et al.: "Host defensive, immunological, and microbiological observations of early-onset periodontitis patient with virus-associated hemophagocytic syndrome" J.Periodontol. 68. 1223-1230 (1997)

Kono, T.、Takikawa, M.、Takashiba, S.、Arai, H.等人：“患有病毒相关噬血细胞综合征的早发性牙周炎患者的宿主防御、免疫学和微生物学观察”J.Periodontol。