The mechanism of the acquired resistance to the anti-proliferative effect of IL-1 on human melanoma cells.

人黑色素瘤细胞系在长时间培养后对人IL-1 α的抗增殖作用产生抗性。利用敏感和抗性克隆，我们研究了细胞获得抗性的机制。抗性细胞组成性产生IL-1 α。IL-1通过自分泌方式诱导耐药细胞产生IL-6，耐药细胞对IL-6的抗增殖作用也具有抵抗性。在一个抗性克隆中，IL-6受体mRNA水平很低，表明获得性抗性是由于缺乏IL-6受体所致。

A human melanoma cell line became resistant to the anti-proliferativr effect of human IL-1 apha after a long period of culture. Using the sensitive and resistant clones, we investigated the mechanism by which the cells acquired the resistance. Tesistant cells produced constitutively IL-1 alpha. The IL-1 induced IL-6 production in the resistant cells by an autocrine manner, and the resistant cells were also resistant to the anti-proliferative effect of IL-6. In one resistant clone, IL-6 receptor mRNA level was quite low, suggesting that the acquired resistance results from the lack of IL-6 receptor.