The mechanism of the acquired resistance to the anti-proliferative effect of IL-1 on human melanoma cells.

IL-1对人黑色素瘤细胞的抗增殖作用获得性抵抗的机制。

• 批准号: 04671364
• 负责人: ONOZAKI Kikuo
• 金额: $ 1.41万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671364/
• 关键词: interleukin 1; cell growth inhibition; melanoma; IL-1Ra; interleukin6; IL-1 receptor; active oxygens; TNF; サイトカイン; マクロファージ

A human melanoma cell line became resistant to the anti-proliferativr effect of human IL-1 apha after a long period of culture. Using the sensitive and resistant clones, we investigated the mechanism by which the cells acquired the resistance. Tesistant cells produced constitutively IL-1 alpha. The IL-1 induced IL-6 production in the resistant cells by an autocrine manner, and the resistant cells were also resistant to the anti-proliferative effect of IL-6. In one resistant clone, IL-6 receptor mRNA level was quite low, suggesting that the acquired resistance results from the lack of IL-6 receptor.

人黑色素瘤细胞系在长时间培养后对人IL-1 α的抗增殖作用产生抗性。利用敏感和抗性克隆，我们研究了细胞获得抗性的机制。抗性细胞组成性产生IL-1 α。IL-1通过自分泌方式诱导耐药细胞产生IL-6，耐药细胞对IL-6的抗增殖作用也具有抵抗性。在一个抗性克隆中，IL-6受体mRNA水平很低，表明获得性抗性是由于缺乏IL-6受体所致。

项目成果

A.Takeuchi,H.Hayashi,Y.Naito,T.Baba,T.Tamatani,K.Onozaki: "Human myelomonocytic cell line THP-1 produces a novel growth promoting factor with a wide target cell spectrum." Cancer Research. (1993)

A.Takeuchi、H.Hayashi、Y.Naito、T.Baba、T.Tamatani、K.Onozaki：“人骨髓单核细胞系 THP-1 产生一种具有广泛靶细胞谱的新型生长促进因子。”

M.Araki, T.Yano, H.Hayashi, T.Takii, K.Suzuki and K.Onozaki: "Eesistance to the anti-ptokiferative effect of IL-1 on human melanoma cell lise is associated with endogenous production of IL-1 and IL-6." Int.J.Caner. 56. 275-280 (1994)

M.Araki、T.Yano、H.Hayashi、T.Takii、K.Suzuki 和 K.Onozaki：“IL-1 对人类黑色素瘤细胞的抗肿瘤作用的存在与 IL-1 的内源性产生有关

K.Onozaki,K.Kato,S.Inui,Y.Takuwa,Y.Akiyama: "Cyclic AMP mimics IL-1 action in augmenting the differ-entiation of a mouse myeloid leukemic cell line(M1)." Journal of Pharmacobio-Dynamics. 15. 491-500 (1992)

K.Onozaki、K.Kato、S.Inui、Y.Takuwa、Y.Akiyama：“环 AMP 模拟 IL-1 作用，增强小鼠骨髓白血病细胞系 (M1) 的分化。”

A.Takeuchi,H.Hayashi,Y.Naito,T.Baba,T.Tamatani,K.Onozaki: "Human myelomonocytic cell line THP-1 produces a novel growth promoting factor with a wide target cell spectrum." Cancer Research. 53. 1871-1876 (1993)

A.Takeuchi、H.Hayashi、Y.Naito、T.Baba、T.Tamatani、K.Onozaki：“人骨髓单核细胞系 THP-1 产生一种具有广泛靶细胞谱的新型生长促进因子。”

A.Takeuchi, H.Hayashi, Y.Naito, T.Baba, T.Tamatani and K.Onozaki: "Human myelomonocytic cell line THP-1 produces a novel growth promoting favtor with a wide target cell spectrum." Cancer Res.53. 1871-1876 (1993)

A.Takeuchi、H.Hayashi、Y.Naito、T.Baba、T.Tamatani 和 K.Onozaki：“人骨髓单核细胞系 THP-1 产生一种具有广泛靶细胞谱的新型生长促进因子。”