Study on the Interleukin 1 Growth-Regulatory Activity.

Interleukin1(IL-1)which is produced by macrophages plays an important role in various host reactions.In this study we we investigated the signal transduction pathway of IL-1 antiproliferative and differentiative activities.1。IL-1 antiproliferative effect in relation to cell cycle.IL-1 inhibits the growth of a human myeloma cell line A375-6。IL-1 antiproliferative effect appeared to be resulted from the prevention of cell movement from G1 to S phase.2。IL-1 antiproliferative and differentiative effects and signal transduction pathway.IL-1 inhibits the growth of a mouse myeloid leukemic cell line(M1)，and in conjunction with other cytokines IL-1 induces M1 cell differentiation into macrophages.Prostaglandin E2，Ca^&lt；2&gt；and PKC did not mediate IL-1effect。In contrast，cAMP exhibited the effect similar to that of IL-1。However，IL-1 failed to increase intracellular cAMP levels in M1 cells.IL-1 also failed to increase cAMP in other cell lines.Therefore，it was suggested that IL-1 provides similar signal as cAMP without increasing intracellular cAMP level.3。Changes in IL-1 levels in human amniotic fluid(AF)with gestational ages and delivery.Since IL-1regulates growth and differentiation of many cell types，we measured the IL-1levels in human AFs which form the intrauterine environment for fetus。IL-1 alpha was present in AF of early gestational ages24weeks，and it increased with gestational ages36weeks.In contrast，IL-1beta was not detectable。However，the content of IL-1 alpha and beta in AF obtained transvaginally during delivery with labor was extremely high.Therefore，IL-1 alpha may relate to fetal growth.IL-1 alpha and beta also may play an important role in occurrence of labor.

Interleukin 1 (IL-1) which is produced by macrophages plays an important role in various host reactions. In this study we we investigated the signal transduction pathway of IL-1 antiproliferative and differentiative activities.1. IL-1 antiproliferative effect in relation to cell cycle. IL-1 inhibits the growth of a human myeloma cell line A375-6. IL-1 antiproliferative effect appeared to be resulted from the prevention of cell movement from G1 to S phase.2. IL-1 antiproliferative and differentiative effects and signal transduction pathway. IL-1 inhibits the growth of a mouse myeloid leukemic cell line (M1), and in conjunction with other cytokines IL-1 induces M1 cell differentiation into macrophages. Prostaglandin E2, Ca^<2+> and PKC did not mediate IL-1 effect. In contrast, cAMP exhibited the effect similar to that of IL-1. However, IL-1 failed to increase intracellular cAMP levels in M1 cells. IL-1 also failed to increase cAMP in other cell lines. Therefore, it was suggested that IL-1 provides similar signal as cAMP without increasing intracellular cAMP level.3. Changes in IL-1 levels in human amniotic fluid (AF) with gestational ages and delivery. Since IL-1 regulates growth and differentiation of many cell types, we measured the IL-1 levels in human AFs which form the intrauterine environment for fetus. IL-1alpha was present in AF of early gestational ages 24 weeks, and it increased with gestational ages 36 weeks. In contrast, ILー1beta was not detectable. However, the content of IL-1alpha and beta in AF obtained transvaginally during delivery with labor was extremely high. Therefore, IL-1alpha may relate to fetal growth. IL-1 alpha and beta also may play an important role in occurrence of labor.