权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on the Interleukin 1 Growth-Regulatory Activity.

白细胞介素1生长调节活性的研究。

基本信息

批准号：
01571233
负责人：
ONOZAKI Kikuo
金额：
$ 1.34万
依托单位：
Fac of Pharmaceutical Sciences, Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571233/
关键词：
interleukin 1 cytokine growth regulation myeloma cell myeloid leukemia cell cell cycle signal transduction amniotic fluid 増殖分化プロスタグランジンE_2 インタ-ロイキン6

项目摘要

Interleukin 1 (IL-1) which is produced by macrophages plays an important role in various host reactions. In this study we we investigated the signal transduction pathway of IL-1 antiproliferative and differentiative activities.1. IL-1 antiproliferative effect in relation to cell cycle. IL-1 inhibits the growth of a human myeloma cell line A375-6. IL-1 antiproliferative effect appeared to be resulted from the prevention of cell movement from G1 to S phase.2. IL-1 antiproliferative and differentiative effects and signal transduction pathway. IL-1 inhibits the growth of a mouse myeloid leukemic cell line (M1), and in conjunction with other cytokines IL-1 induces M1 cell differentiation into macrophages. Prostaglandin E2, Ca^<2+> and PKC did not mediate IL-1 effect. In contrast, cAMP exhibited the effect similar to that of IL-1. However, IL-1 failed to increase intracellular cAMP levels in M1 cells. IL-1 also failed to increase cAMP in other cell lines. Therefore, it was suggested that IL-1 provides similar signal as cAMP without increasing intracellular cAMP level.3. Changes in IL-1 levels in human amniotic fluid (AF) with gestational ages and delivery. Since IL-1 regulates growth and differentiation of many cell types, we measured the IL-1 levels in human AFs which form the intrauterine environment for fetus. IL-1alpha was present in AF of early gestational ages 24 weeks, and it increased with gestational ages 36 weeks. In contrast, ILー1beta was not detectable. However, the content of IL-1alpha and beta in AF obtained transvaginally during delivery with labor was extremely high. Therefore, IL-1alpha may relate to fetal growth. IL-1 alpha and beta also may play an important role in occurrence of labor.

巨噬细胞产生的白介素1(IL-1)在多种宿主反应中起重要作用。在本研究中，我们研究了IL-1抗增殖和分化活性的信号转导途径。IL-1抗增殖作用与细胞周期的关系。IL-1抑制人骨髓瘤细胞系A375-6的生长。结论：1.IL-1的抗增殖作用可能是通过阻止细胞从G1期向S期移动所致。IL-1的抗增殖分化作用及其信号转导途径。IL-1抑制小鼠髓系白血病细胞系(M1)的生长，并与其他细胞因子联合诱导M1细胞向巨噬细胞分化。前列腺素E_2、Ca~(2+)和PKC不能介导IL-1的作用。相反，cAMP表现出与IL-1相似的作用。然而，IL-1不能增加M1细胞内cAMP水平。IL-1也不能增加其他细胞系中cAMP的含量。因此，提示IL-1在不增加细胞内cAMP水平的情况下提供与cAMP类似的信号。人羊水中IL-1水平与胎龄和分娩的关系由于IL-1调节多种细胞类型的生长和分化，因此我们检测了构成胎儿宫内环境的人AFS中的IL-1水平。IL-1α在早期胎龄24周的房颤中呈阳性表达，随着胎龄36周的增加，IL-1α的表达逐渐增加。相反，未检测到IL-ー-1β。然而，在分娩过程中经阴道获得的房颤患者的IL-1α和β含量极高。因此，IL-1α可能与胎儿生长发育有关。IL-1α和β也可能在分娩过程中发挥重要作用。