New hepatocarcinogen, fumonisins : food contamination and the distribution of fumonishin-producing fungi

新的肝癌物质伏马菌素：食品污染和产伏马菌素的真菌分布

• 批准号: 04671374
• 负责人: UENO Yoshio
• 金额: $ 1.34万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671374/
• 关键词: Fumonisin B1; Fumonisin B2; Corn; Hepatocarcinogen; Fusarium moniliforme; Japan; Asia; ネパール; 中国; タイ; マレーシア; フモニシン; カビ毒; 飼料汚染; フザリウム菌

Natural occurrence of fumonisin B1 and B2, a promoter for hepatocarci nogenesis, was investigated in corn and corn-based products sampled in Japan, Nepal, China, Thailand and Malaysia by high-performance liquid chromatographic method. In 9 imported corn kernel and 6 gluten feed samples, fumonisin B1 was detected in 8 corn (0.6-4.1 mug/g) and all gluten feed (0.3-2.4 mug/g) samples, while fumonisin B2 was found in the same corn (0.3-10.2 mug/g) and 3 gluten feed (0.8-8.5 mug/g) samples. ELISA analysis also revealed the contamination of aflatoxin B1 in 2 corn and all gluten feed samples along with fumonisins. In 17 samples of corn grits, 14 and 5 samples were contaminated with fumonisin B1 and B2, with the maximum levels of 2.6 and 2.8 mug/g, respectively. As for corn-based foodstuffs marketed in Japan, no significant contamination of fumonisins was observed. Among 24 samples of corn kernels in Nepal, 12 and 7 samples were positive for fumonishin B1 and B2, and averaged to 0.6 and 1.6 mug/g, respectively. The maximal contents were counted to 4.6 and 5.5 mug/g in one same sample respectively. In corn samples harvested at Shanghai and Beijing in China, fumonisin B1 and B2 were detected in various concentrations. Thailand samples were positive for fumonisin B1 and B2 at levels ranging from 0.3 to 1.8 and 0.1 to 0.4 mug/g, respectively. In Malaysia, a few samples were positive for fumonisin B1 and B2. Mycological survey has revealed the presence of fumonisin-producing fungus in a crop field in Japan. These findings have firstly demonstrated the contamination of fumonisins with high levels in corn and corn-based products in Asian contries. Natural co-occurrence of fumonisins and aflatoxin B1 was also detected in raw materials for mixed feed.

采用高效液相色谱法对日本、尼泊尔、中国、泰国和马来西亚的玉米和玉米制品中伏马菌素B1和B2（肝癌发生的促进剂）的天然存在进行了研究。在9个进口玉米粒和6个面筋饲料样品中，在8个玉米（0.6-4.1 mug/g）和所有面筋饲料（0.3-2.4 mug/g）样品中检测到伏马菌素B1，而在同一玉米（0.3-10.2 mug/g）和3个面筋饲料（0.8-8.5 mug/g）样品中检测到伏马菌素B2。ELISA分析还显示，黄曲霉毒素B1污染的2个玉米和所有面筋饲料样品沿着与伏马菌素。在17个玉米粉样品中，14个和5个样品被伏马菌素B1和B2污染，最高水平分别为2.6和2.8 mug/g。至于在日本销售的玉米食品，没有观察到伏马菌素的严重污染。在尼泊尔的24个玉米粒样品中，12个和7个样品对伏马菌素B1和B2呈阳性，平均值分别为0.6和1.6 μ g/g。同一样品中最大含量分别为4.6和5.5 μ g/g。在中国上海和北京收获的玉米样品中，检测到不同浓度的伏马菌素B1和B2。泰国样本对伏马菌素B1和B2呈阳性，水平分别为0.3至1.8和0.1至0.4微克/克。在马来西亚，一些样本对伏马菌素B1和B2呈阳性。真菌学调查显示，在日本的农田中存在产生伏马菌素的真菌。这些发现首次证实了亚洲国家玉米及其制品中伏马菌素的高水平污染。在混合饲料原料中也检测到伏马菌素和黄曲霉毒素B1的自然共存。

项目成果

Ueno, Y., Aoyama, S., Sugiura, Y., Wang, D-S., Lee, U-S., Hirooka, E.Y., Hara, S., Karki, T., Chen, G.and Yu, S-Z.: "A limited survey of fumonisins in corn and corn-based products in Asian counties" Vol.9. 27-34 (1993)

Ueno, Y.、Aoyama, S.、Sugiura, Y.、Wang, D-S.、Lee, U-S.、Hirooka, E.Y.、Hara, S.、Karki, T.、Chen, G. 和 Yu, S-Z.：“

Ueno, Y., Aoyama, S., Sugiura, Y., Wang, D-S., Lee, U-S., Hirooka, E.Y., Hara, S., Karki, T., Chen, G.and Yu, S-Z.: "A limited survey of fumonisins in corn and corn-based products in Asian counties" Vol.9. 27-34 (1993)Mycotoxin Research.

Ueno, Y.、Aoyama, S.、Sugiura, Y.、Wang, D-S.、Lee, U-S.、Hirooka, E.Y.、Hara, S.、Karki, T.、Chen, G. 和 Yu, S-Z.：“

Ueno,Y.et al.: "A limited survey of fumonisins in corn and corn-based products in Asian countries" Mycotoxin Research. 9. 27-34 (1993)

Ueno,Y.et al.：“亚洲国家玉米和玉米制品中伏马菌素的有限调查”霉菌毒素研究。

Ueno Y: "A limited survey of fumonisins in corn and corn-based products in Asian countries" Mycotoxin Research.

Ueno Y：“对亚洲国家玉米和玉米制品中伏马菌素的有限调查”霉菌毒素研究。