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Chemotaxonomy of toxigenic Fusarium on the base of DNA homology

基于DNA同源性的产毒镰刀菌化学分类学

基本信息

批准号：
02807206
负责人：
UENO Yoshio
金额：
$ 0.9万
依托单位：
Science University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Plant pathogenic fungi, Fusaria, are distributed through the world and known to produce several mycotoxins. The consumption of cereals contaminated with mycotoxins cause several mycotoxicoses in human and livestocks. Classification of Fusarium is based on their morphological criteria. However, most of Fusarium researchers have been encountered difficulties on the identification of Fusarium isolates. This is because of lack of morphological stability of species within the group.In this study, two molecular biological approaches were applied for the identification and classification of 9 toxigenic Fusarium species.(1) DNA homology test : Each denatured genome DNA was hybridized with the biotinlabeled DNA probe of test strain. Homology values of 9 Fusarium species against three different test strains ranged 50 to 119% in average, and more than 90% within the same species. However, over 100% of homology value was shown between different species. Further studies were required.(2) Application RFLP patterns : By Southern blot analysis, two 32p-labeled fragments, 1.1 or 1.7kb, generated from a Fn-2B strain of Fusarium, were hybridized with other Fusaria DNAs digested with three different restriction enzymes. The 1.1kb fragment showed similar patterns within the same species, and relatively different patterns among the different species. No critical data with the 1.7kb fragment was obtained.In conclusion, although the hybridization patterns of total DNA against 1.1kb fragment provides an alternative method to differentiate two closely related species of Fusarium, we could not determine whether this may unequivocally identify all isolates or not. Neverthless, RFLP patterns provide an useful tool to distinguish the important toxgenic Fusarium species.

植物病原真菌镰刀菌(Fusaria)广泛分布于世界各地，可产生多种真菌毒素。食用被霉菌毒素污染的谷物会对人和牲畜造成几种霉菌中毒。镰刀菌属的分类是基于它们的形态标准。然而，大多数镰刀菌研究人员在镰刀菌分离物的鉴定上遇到了困难。本研究采用两种分子生物学方法对9种产毒镰刀菌进行鉴定和分类：(1)DNA同源性检验：将变性的基因组DNA与供试菌株的生物标记DNA探针杂交。9个镰刀菌对3个不同供试菌株的同源性平均在50%~119%之间，同种间的同源性在90%以上。不同物种间的同源性均在100%以上。应用RFLP图谱：通过Southern印迹分析，将FN-2B菌株产生的1.1或1.7kb的两个32P标记片段与三种不同限制性内切酶消化的其他镰刀菌DNA杂交。1.1kb片段在同一物种内表现出相似的模式，在不同物种之间表现出相对不同的模式。尽管总DNA与1.1kb片段的杂交模式提供了一种区分两个密切相关的镰刀菌物种的替代方法，但我们不能确定这是否可以明确地识别所有的分离物。然而，RFLP图谱为区分重要的致毒镰刀菌种提供了一个有用的工具。