Evaluation Of Clinical Laboratory Test By Using Quartz Crystal Resonator

研究了石英晶体粘度传感器定量血浆纤维蛋白原浓度的方法。采用AT切晶体作为粘度传感器，在液相中具有井型池。在石英晶体表面预涂纤维蛋白原以保护血浆中内源性纤维蛋白原的结合，在反应混合液中加入凝血酶，用井型池、振荡器和频率计数器监测共振频率的变化。该方法简便、快速、专属性强，在较低的范围内具有较高的准确度，在50-400 mg/dl范围内线性关系良好，可用于测定纤维蛋白原的含量。本法与常规方法比较，相关系数γ =0.702，回归曲线Y=1.89X（本法）-110.21（n=20），健康成人血浆纤维蛋白原浓度的参考范围为170-390 mg/dl。血浆中的重现性（CV）在2.29%-7.97%之间。初步研究表明，石英晶体谐振器是有用的，用于测量血浆凝固因子在液相中的凝胶化。

Quantification of plasma fibrinogen concentration by quartz crystal viscosity sensor was investigated. At-cut crystal was used as viscosity sensor with a well-type cell in a liquid phase. The surface of quartz crystal was precoated with fibrinogen to protect the bonding of endogeneous fibrinogen in the plasma.The assay was initiated by the addition of thrambin into reaction mixture.The resonant frequency change was monitored by using a well-type cell, oscillator and frequency counter. The resonant frequency decrease due to the increase of viscosity by gelation of fibrinogen converted to fibrin.The proposed method is simple, rapid, specific and accurate at the lower range.Results obtained in proposed system showed good linearity over range 50-400 mg/dl. The comparison of proposed method with routine method used yielded a correlation coefficient ; gamma=0.702 and regression curve ; Y=1.89X(proposed method) - 110.21(n=20).Reference interval of plasma fibrinogen concentration from healty adults has in the range of 170-390 mg/dl. Reproducibilities(CVs) was between 2.29% and 7.97% in plasma. The preliminary studies suggested that a quartz crystal resonator is useful for the measurement of gelation of plasma coagulation factors in the liquid phase.