Evaluation Of Clinical Laboratory Test By Using Quartz Crystal Resonator

使用石英晶体谐振器进行临床实验室测试的评价

• 批准号: 04671437
• 负责人: OKABE Hiroaki
• 金额: $ 1.34万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671437/
• 关键词: Quartz Crystal Resonator; Oscillator; Blood Coagulation Factor; Fibrinogen; Thrambin; Piezoelectric Sensor; Viscosity

Quantification of plasma fibrinogen concentration by quartz crystal viscosity sensor was investigated. At-cut crystal was used as viscosity sensor with a well-type cell in a liquid phase. The surface of quartz crystal was precoated with fibrinogen to protect the bonding of endogeneous fibrinogen in the plasma.The assay was initiated by the addition of thrambin into reaction mixture.The resonant frequency change was monitored by using a well-type cell, oscillator and frequency counter. The resonant frequency decrease due to the increase of viscosity by gelation of fibrinogen converted to fibrin.The proposed method is simple, rapid, specific and accurate at the lower range.Results obtained in proposed system showed good linearity over range 50-400 mg/dl. The comparison of proposed method with routine method used yielded a correlation coefficient ; gamma=0.702 and regression curve ; Y=1.89X(proposed method) - 110.21(n=20).Reference interval of plasma fibrinogen concentration from healty adults has in the range of 170-390 mg/dl. Reproducibilities(CVs) was between 2.29% and 7.97% in plasma. The preliminary studies suggested that a quartz crystal resonator is useful for the measurement of gelation of plasma coagulation factors in the liquid phase.

研究了石英晶体粘度传感器定量血浆纤维蛋白原浓度的方法。采用AT切晶体作为粘度传感器，在液相中具有井型池。在石英晶体表面预涂纤维蛋白原以保护血浆中内源性纤维蛋白原的结合，在反应混合液中加入凝血酶，用井型池、振荡器和频率计数器监测共振频率的变化。该方法简便、快速、专属性强，在较低的范围内具有较高的准确度，在50-400 mg/dl范围内线性关系良好，可用于测定纤维蛋白原的含量。本法与常规方法比较，相关系数γ =0.702，回归曲线Y=1.89X（本法）-110.21（n=20），健康成人血浆纤维蛋白原浓度的参考范围为170-390 mg/dl。血浆中的重现性（CV）在2.29%-7.97%之间。初步研究表明，石英晶体谐振器是有用的，用于测量血浆凝固因子在液相中的凝胶化。

项目成果

宇治義則、岡嶋研二他: "水晶振動子を用いた血液凝固因子の測定" 日本臨床化学会九州支部会誌. 4,(in press). (1994)

Yoshinori Uji、Kenji Okajima 等：“使用石英晶体振荡器测量血液凝固因子”，日本临床化学学会九州分会杂志 4，（出版中）。

Yoshinori UJI et al: "Measurement of blood coagulation factors by quartz crystal resonator" Kyushu J.Clinical Chemistry. 4. (1994)

Yoshinori UJI 等：“石英晶体谐振器测量凝血因子”九州 J.临床化学。

宇治義則、岡部紘明、岡嶋研二、杉内博幸、中川富美子: "水晶振動子を用いた血液凝固因子の測定" 日本臨床化学会九州支部会誌. 4(校正中). (1994)

Yoshinori Uji、Hiroaki Okabe、Kenji Okajima、Hiroyuki Sugiuchi、Fumiko Nakakawa：“使用晶体振荡器测量血液凝固因子”日本临床化学学会九州分会杂志 4（修订中）。

Hiroaki OKABE et al: "Proceedings on XIII Conference of Clinical Analysis ; Evaluation of Clinical Laboratory Tests by Using Quartz Crystal Resonator." Committee of Clinical Laboratory Analysis. Vol.4. (1994)

Hiroaki OKABE 等人：“第十三届临床分析会议论文集；使用石英晶体谐振器评估临床实验室测试”。

岡部紘明、岡嶋研二、中川富美子、宇治義則、杉内博幸: "第13回臨床分析研究会要旨集「水晶振動子を用いた臨床検査法の開発」Vol.4" 臨床分析研究会事務局編(国立循環器病センター臨床検査部), 150 (1994)

Hiroaki Okabe、Kenji Okajima、Fumiko Nakakawa、Yoshinori Uji、Hiroyuki Sugiuchi：“第 13 届临床分析研究小组摘要‘使用石英晶体的临床测试方法的开发’第 4 卷”由临床分析研究小组秘书处编辑（国家脑科学研究会）和心血管中心临床检验部），150（1994）