Photodynamic therapy for Rheumatoid Arthritis

类风湿关节炎的光动力疗法

• 批准号: 05671237
• 负责人: OTSUJI Takaaki
• 金额: $ 1.54万
• 依托单位: Tokyo Women's Medical Cllege
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671237/
• 关键词: PHOTOCHEMOTHERAPY; ARTHRITIS,Rheumatoid; RAT; mono-L-aspartyl chlorin e6; 滑膜組織; 光化学療法; アジュバント関節炎; ラット

We have been researching photodynamic therapy (PDT) of rheumatoid arthritis with the support of the GRANT-IN-AID FOR SCIENTIFIC RESEARCH since 1992. We have been experimenting on arthritic rat animal models with NPe6 (MEIJI) as a photosensitizer and Panasonic diode laser system as a laser light source. The NPe6 showed high accumulation in the synovial membrane of the adjuvant induced arthritis of the rat after intravenous injection. We could observe a hemorrhogic reaction in the proliferative synovial membrane immediately after the direct PDT by arthrotomy, and a necrotic synovial change without cartilage or tendon damage after 7 days of the PDT.But when the PDT was done with intrarticular injection of the sensitizer and through the skin irradiation of the laser light, an unexpected high level of acute lethality SUand the severely damaged cartilage and bone in the treated joint was observed. Photodynamic therapy may show promise in the treatment of rheumatoid arthritis but the development of an elaborate method is mandatory before clinical trials.

自1992年以来，我们一直在研究类风湿性关节炎的光动力疗法（PDT），并得到了科学研究补助金的支持。我们以NPe6 （MEIJI）作为光敏剂，以松下二极管激光系统作为激光光源，在关节炎大鼠动物模型上进行了实验。静脉注射后，NPe6在佐剂诱导的大鼠关节炎滑膜中有较高的蓄积。通过关节切开术直接行PDT后，我们可以观察到增生性滑膜立即出现出血反应，PDT后7天滑膜坏死改变，无软骨或肌腱损伤。但是，当PDT通过关节内注射致敏剂和通过激光照射皮肤时，观察到意想不到的高急性致死率和治疗关节中严重受损的软骨和骨。光动力疗法在类风湿关节炎的治疗中可能显示出希望，但在临床试验之前必须开发一种详细的方法。