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STRATEGY FOR ENVIRONMENTAL ADAPTATION IN AQUATIC PLANTS

水生植物的环境适应策略

基本信息

批准号：
05804046
负责人：
ISHIZAWA Kimiharu
金额：
$ 1.22万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05804046/
关键词：
Aquatic plants Cattail (typha latiforia L.)Anaerobic tolerance Environmental adaptation alcohol dehydrogenase Arrowhead (Sagittaria pygmaea Miq.)Protein synthesis Anaerobiosis ガマ Typha latifoloa L.Typha latifolia poly(A)^+RNA

项目摘要

1. We collected cattail plants (Typha latifolia L.) from the natural habitat and also our green house. The arrowhead (Sagittaria pygmaea Miq.) plants were supplied from the Weed Science Center of Utsunoimiya University. We made a continuous N_2 gas-flow system, in which pure N_2 gas from a cylinder was introduce through a pressure regulater and a flow controller valve in order to treat the plants with low O_2 concentratiions less than 100ppm.2. The ADH activity of cattail buds increased significantly within 6 h of anoxic treatment and kept a maximal value for 10 days. Moreover, the elongation of arrowhead bud was promoted under anoxia with concomitant increase in the activity of ADH.The results suggested that increase in ADH activity under anoxia is related to the tolerance to anoxia.3. We analyzed in vivo translation products of cattail or arrowhead buds by 2-D electrophoresis analysis. The effects of anoxic treatment on protein synthesis in cattail buts were quite different from that in arrowhead buds. Synthesis of aerobical polypeptides in cattail buts were almost stopped under anaerobic conditions for 2 hours. Synthesis of new polypeptides stated after 8hours of anaerobic treatment. In arrowhead tubers, on the other hand, many polypeptides synthesized aerobically were translated unde anaerobic conditions. The active translation may be related for the promotion of leaf elongation under anoxia.

1.以香蒲（Typha latifolia L.）还有我们的绿色房子。慈姑（Sagittaria pygmaea Miq.）植物由Utsunoimiya大学杂草科学中心提供。我们制作了一个连续的N_2气流系统，通过一个压力调节器和一个流量控制阀从一个钢瓶中引入纯N_2气体，以处理低O_2浓度小于100ppm的植物.缺氧处理6h内，蒲芽的ADH活性明显升高，并在10d内保持最大值。此外，缺氧促进慈姑芽的伸长，同时也促进了ADH活性的提高，说明缺氧条件下ADH活性的提高与慈姑芽的耐缺氧能力有关.我们用二维电泳分析了香蒲或慈姑芽的体内翻译产物。缺氧处理对香蒲芽蛋白质合成的影响与慈姑芽有很大不同。香蒲需氧多肽的合成在厌氧条件下2h几乎停止。厌氧处理8小时后，新的多肽合成。在慈姑块茎，另一方面，许多多肽有氧合成的厌氧条件下翻译。这种主动翻译可能与促进缺氧条件下叶片伸长有关。