Effects of prostaglandin and noradrenalin on sleep-related neurons in the preoptic area.

前列腺素和去甲肾上腺素对视前区睡眠相关神经元的影响。

• 批准号: 05807006
• 负责人: OSAKA Toshimasa
• 金额: $ 0.96万
• 依托单位: OSAKA BIOSCIENCE INSTITUTE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807006/
• 关键词: Sleep; Neuron; Prostaglandin; Noradrenaline; Preoptic area; ラット; 多連微小ピペット; クロニジン

Electrical stimulation of noradrenergic cells in the locus coeruleus and ventrolateral medulla inhibited sleep-active neurons and excited waking-active neurons in the preoptic area of the rat. Noradrenaline applied through a multibarrel micropipette also inhibited sleep-active neurons and excited waking-active neurons. Application of antagonists and agonists for adrenoceptors revealed that inhibition was mediated by alpha_2-and excitation by alpha_1-receptors.During wakefulness, prostaglandin D_2 (PGD_2) excited sleep-active neurons and inhibited waking-active neurons. Continuous application of PGD_2 attenuated neuron activity during wakefulness. However, PGD_2 was less effective in eliciting a response during sleep. PGD_2 also attenuated noradrenaline-induced responses of sleep-related neurons.These results suggest (1) noradrenaline promotes wakefulness by inhibiting sleep-active neurons through alpha_2-receptors and by exciting waking-active neurons through alpha_1-receptors, (2) PGD_2 promotes sleep by exciting sleep-active neurons and by inhibiting waking-active neurons, (3) most important action of PGD_2 is the attenuation of activity during wakefulness, and (4) PGD_2 attenuates the noradrenaline-mediated arousal influence.

电刺激蓝斑和延髓腹外侧区去甲肾上腺素能神经元抑制大鼠视前区睡眠激活神经元和觉醒激活神经元。通过多管微吸管施加去甲肾上腺素也抑制睡眠活跃神经元和兴奋清醒活跃神经元。肾上腺素能受体拮抗剂和激动剂的应用表明，抑制是由α_2受体和α_1受体介导的，清醒时前列腺素D_2(PGD_2)兴奋睡眠活动神经元，抑制觉醒活动神经元。持续应用PGD_2可减弱清醒状态下神经元的活动。然而，PGD_2在睡眠中引起反应的效果较差。这些结果表明：(1)去甲肾上腺素通过α_2受体抑制睡眠激活神经元，通过α_1受体兴奋唤醒神经元，从而促进觉醒；(2)PGD_2通过兴奋睡眠激活神经元和抑制觉醒激活神经元而促进睡眠；(3)PGD_2最重要的作用是在觉醒时减弱活动，以及(4)PGD_2减弱去甲肾上腺素介导的唤醒影响。

项目成果

Osaka,T.: "Noradrenaline inhibits preoptic sleep-active neurons through α_2-receptors in the rat." Neuroscience Research. (1995)

Osaka, T.：“去甲肾上腺素通过大鼠的 α_2 受体抑制视前睡眠活跃神经元。”（神经科学研究）。

Toshimasa Osaka: "Norddrenaline inhibits preoptic sleep-actiue neurons through α2-receptors in the rat" Neuroscience Research. (1995)

Toshimasa Osaka：“去甲肾上腺素通过大鼠的 α2 受体抑制视前睡眠激活神经元”神经科学研究（1995）。

Toshimasa Osaka: "Noradrenergic inputs to sleep‐nelated neurans in the preoptic area from the locus coeruleus and the ventrolateral medalla in the rat" Neuroscience Research. (1994)

Toshimasa Osaka：“来自大鼠蓝斑和腹外侧奖章的视前区睡眠相关神经元的去甲肾上腺素能输入”神经科学研究（1994）。

Matsumura,H.: "Prostaglandin D_2-sensitive,sleep-promoting zone defined in the ventral surface of the rostral basal forebrain." Proceedings of the National Academy of Sciences USA. 91. 11998-12002 (1994)

Matsumura,H.：“前列腺素 D_2 敏感、促进睡眠的区域定义在前脑头端基底腹侧表面。”

Osaka, T.and Matsumura, H.: "Noradrenaline inhibits preoptic sleep-active neurons through alpha_2-receptors in the rat." Neuroscience Research.(in press).

Osaka, T. 和 Matsumura, H.：“去甲肾上腺素通过大鼠的 α_2 受体抑制视前睡眠活跃神经元。”