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Molecular Analysis of RT-like gene which is newly found in Escherichia coli.

大肠杆菌中新发现的RT样基因的分子分析。

基本信息

批准号：
05808063
负责人：
AMEMURA Mitsuko
金额：
$ 1.22万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05808063/
关键词：
Escherichia coli K-12 Escherichia coli C Escherichia coli B Reverse Transcriptase Retrovirus IS 5 IS 629 group II intron レトロトランスポゾン系統樹進化グルタチオンSトランスフェラーゼ融合遺伝子トランスポジション

项目摘要

Many genetic elements from a wide variety of organisms have been shown to contain open-reading frames encoding proteins that are similar sequence to retroviral reverse transcriptases. We isolated a new reverse transcriptase (RT) -like gene from Escherichia coli K-12 and it is located at 6 min on the E.coli genetic map. The gene encodes a protein that consists of 322 amino acids, which has conserved domains I to VII that could be identified in all RT-like sequences. Downstream from the conserved domain VII,IS 5 insertion element was found. Amino acid alignment of the RT-like proteins and various kinds of RTs shows that the RT-like protein belongs to rather the RTs found in certain fungal group II mitochondorial introns than the msDNA related RTs found in enteric bacteria. The gene also exists in E.coli C with multicopy and Shigella dysenteriae type I strain, but dose not exist in E.coli B and othe bacterial species investigated. One of the RT-genes derived from E.coli C was cloned and sequenced. It encodes 479 amino acides. RT-like protein in E.coli C is larger than RT-like protein in E.coli K-12. The amino acid sequences from 1 to 322 of both RTs are the same. Downstream of the conserved RT domains, we found IS 629 insertion element which is thought to be derived from Shigella sonnei. Interestingly we found 45 bp long sequence homologous to mitochondorial cytochrome oxidase intron2 of Saccharomyces cerevisiae between terminal codon of RT gene and IS 629.

来自多种生物体的许多遗传元件已被证明含有编码与逆转录病毒逆转录酶序列相似的蛋白质的开放阅读框。我们从大肠杆菌K-12中分离到一个新的逆转录酶（RT）样基因，它位于大肠杆菌遗传图谱上的6 min处。该基因编码一种由322个氨基酸组成的蛋白质，其具有可以在所有RT样序列中鉴定的保守结构域I至VII。RT样蛋白和各种RT的氨基酸序列比对表明，RT样蛋白与某些真菌Ⅱ类线粒体内含子中的RT相似，而与肠道细菌中msDNA相关的RT相似。该基因也存在于多拷贝大肠杆菌C和痢疾杆菌I型菌株中，而不存在于大肠杆菌B和其他被调查的细菌种中。克隆并测序了来源于大肠杆菌C的RT基因之一。它编码479个氨基酸。大肠杆菌C中的RT样蛋白比大肠杆菌K-12中的RT样蛋白大。两种RT的1至322的氨基酸序列相同。在保守的RT结构域下游，我们发现了IS 629插入元件，该元件被认为是宋内志贺菌的衍生物。有趣的是，我们在RT基因的末端密码子和IS 629之间发现了与酿酒酵母线粒体细胞色素氧化酶内含子2同源的45 bp长的序列。