Myosin Light Chaiu Kinase in sea urchin egg

海胆蛋中的肌球蛋白轻 Chaiu 激酶

• 批准号: 05808066
• 负责人: HOSOYA Hiroshi
• 金额: $ 1.22万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05808066/
• 关键词: Cell cycle; MLCK; Myosin; myonin regulatory light chaiu, phoshorylation; myosir light chain kinase; ミオシン調節軽鎖; ミオシン軽鎮キナーゼ; Cell cycle; 細胞分裂; ミオシン軽鎖; 細胞周期; cdc2キナーゼ; アクチン; 細胞骨格

We have analyzed myosin light chain kinase (MLCK) activities in unfertilized and fertilized egg extracts from sea urchin, Hemicentrotus pulcherrimus. Total kinase activities that phosphorylates regulatory light chain of mysion-II (MLC) are higher in unfertilized egg extracts than in fertilized egg extracts in vitro. Phosphopeptide mapping analysis shows that the kinase activities in unfertilized eggs phosphorylate MLC both at the sites which MLCK purified from chicken gizzard (cgMLCK) and protein kinase C (PKC) phosphorylate, respectively. In metaphase, the kinase activity that phosphorylates MLC at cgMLCK sites is decreased, but the kinase activity that phosphorylates MLC at PKC sites is increased. Fractionation of undfertilized egg extracts using a gel filtration chromatography resulted in two peaks (termed fraction I and fraction II) of sea urchin MLCK activities. Prosphopeptide mapping analsis shows that both kinases phosphorylate MLC at cgMLCK sites but one is calciumcalmodulin dependent MLCK and the other is calciumcalmodulin independent MLCK,respectively.

本文分析了海胆（hemicentrrotus pulcherrimus）未受精卵和受精卵提取物中肌球蛋白轻链激酶（MLCK）的活性。体外实验表明，未受精卵提取物磷酸化myion - ii调节轻链（MLC）的总激酶活性高于受精卵提取物。磷酸化肽图谱分析表明，未受精卵的激酶活性使MLC磷酸化，分别位于MLCK从鸡胗中纯化的位点（cgMLCK）和PKC蛋白激酶磷酸化的位点。在中期，在cgMLCK位点磷酸化MLC的激酶活性降低，但在PKC位点磷酸化MLC的激酶活性增加。用凝胶过滤层析法对未受精卵提取物进行分离，得到海胆MLCK活性的两个峰（称为分数I和分数II）。Prosphopeptide定位分析表明，两种激酶都在cgMLCK位点磷酸化MLC，但一种是钙钙调素依赖性MLCK，另一种是钙钙调素非依赖性MLCK。

项目成果

細谷浩史: "Annual Review細胞生物学" 中外医学社, 138-144 (1993)

Hiroshi Hosoya：“细胞生物学年度评论”Chugai Igakusha，138-144（1993）

H.Hosoya, T.Matsuoka, N.Hosoya, T.Takahashi, and T.Kosoka: "Prosence of a Tetrahymena growth promoting activity in fetal bovine serum." Develop.Growth.Differ.(in press). (1995)

H.Hosoya、T.Matsuoka、N.Hosoya、T.Takahashi 和 T.Kosoka：“胎牛血清中存在四膜虫生长促进活性。”

H.Hosoya et al: "Growth promoting activity for Tetrahymena in fetal bovine serum" Develop.Growth Differ.,. in press. (1995)

H.Hosoya 等人：“胎牛血清中四膜虫的生长促进活性”Develop.Growth Differ.，。

H.Hosoya et al: "Aposymibitotic strains of the green paramecium Paramecium bursar ia produced by exposure of the herbicide paraquat" Europ.J.Protist.in press. (1995)

H.Hosoya 等人：“通过接触除草剂百草枯产生的绿色草履虫 Paramecium bursar ia 的无对称菌株”Europ.J.Protist.in press。

H.Hosoya et al: "Mechanism regulating cytokinesis: roles of myosin light chain kinase" Zool.Sci.,. 11. 507-510 (1994)

H.Hosoya 等人：“调节胞质分裂的机制：肌球蛋白轻链激酶的作用”Zool.Sci.，。