Roles of MRLC kinase during cytokinesis

MRLC 激酶在胞质分裂过程中的作用

• 批准号: 09480197
• 负责人: HOSOYA Hiroshi
• 金额: $ 4.03万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480197/
• 关键词: Myosin II; MLCK; phosphorylation; Myosin; HeLa Cell; Cell Division; Cytokinesis; 細胞管分裂; ミオシン; MeLa細胞; 細胞質分裂

A novel myosin light chain kinase cDNA was isolated from HeLa cells cDNA library. The deduced amino acid sequence was identical to that of zipper interacting protein kinase (ZIPK) which mediated apoptosis (Kawai et. al. Mcl. Cell. Bxol. 18, 1642-1651, 1998). Here we found that HeLa ZIPK phosphorylated myosin II regulatory light chain. (MRLC) at both serine 19 and threonine 18 in ca^2^+/calmodulin independent manner. Phosphorylation of myosin II by HeLa ZIPK resulted in activation of actin-activated MgATPase activity of myosin II.HeLa ZIPK is the first kinase from nonmuscle cells that phosphorylates MRLC at two sites.

从HeLa细胞的cDNA文库中克隆到一个新的肌球蛋白轻链激酶基因。推导的氨基酸序列与介导细胞凋亡的拉链相互作用蛋白激酶(ZIPK)的氨基酸序列一致。艾尔MCL。牢房。Bxol。18,1642-1651,1998)。在这里我们发现HeLa ZIPK磷酸化肌球蛋白II调节轻链。(MRLC)在丝氨酸19和苏氨酸18以ca^2^+/钙调蛋白不依赖的方式表达。肌球蛋白II被HeLa ZIPK磷酸化后，肌球蛋白II的肌动蛋白激活的镁ATPase活性被激活。HeLa ZIPK是第一个在两个位点磷酸化MRLC的非肌肉细胞中的激酶。

项目成果

M-A.Okubo: "A monoclonal antibody,mH1,specifically recognizes microvilli of dividing HeLa cells." Tiss.Cult.Res.Comm.(in press). (1999)

M-A.Okubo：“单克隆抗体 mH1 可以特异性识别分裂的 HeLa 细胞的微绒毛。”

M.Murata-Hori: "Singly phosphorylated myosin II regulatory light chain is localized in the cleavage furrow of dividing BeLa cells" Biomed.Res.19. 111-115 (1998)

M.Murata-Hori：“单磷酸化肌球蛋白 II 调节轻链位于分裂 BeLa 细胞的裂解沟中”Biomed.Res.19。

S.Komatsu, M.Murata-Hori, 他: "Involvement of p34cdc2 kinase from sea urchin Hemicentrotus pulcherrimus in the phosphorylation of myosin II regulatory light chain in the metaphase extract." Gene. 198. 359-365 (1997)

S.Komatsu、M.Murata-Hori 等人：“来自海胆 Hemicentrotus pulcherrimus 的 p34cdc2 激酶参与中期提取物中肌球蛋白 II 调节轻链的磷酸化”。 198. 359-365 (1997)

T.Takahashi, M.Okubo, and H.Hosoya: "Regional differentiation of cortical structures and their reorganization during cell division in Paramecium trichium." J.Euk.Microbiol.45. 369-380 (1998)

T.Takahashi、M.Okubo 和 H.Hosoya：“草履虫细胞分裂过程中皮质结构的区域分化及其重组。”

M.Murata-Hori, F.Suizu, T.Iwasaki, A.Kikuchi, and H.Hosoya: "ZIP Kinase identified as a novel myosin regulatory light chain kinase in HeLa cells." FEBS lett.(in press). (1999)

M.Murata-Hori、F.Suizu、T.Iwasaki、A.Kikuchi 和 H.Hosoya：“ZIP 激酶被鉴定为 HeLa 细胞中的一种新型肌球蛋白调节轻链激酶。”