Molecular physiology for the mode of action on senescence promoting effects of jasmonic acid

茉莉酸促衰老作用机制的分子生理学

• 批准号: 05834013
• 负责人: UEDA Junichi
• 金额: $ 1.47万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05834013/
• 关键词: Jasmonic acid; Senescence; Phaseolus vulgaris L.; Abscission; Cell wall polysaccharide; Cellulase; オートムギ

Jasmonic acid (JA) and methyl jasmonate (JA-Me) at more than 1muM promoted the abscission of bean (Phaseolus vulgaris L.cv.Masterpiece) petiole explants in the dark and the light. JA and JA-Me did not promote ethylene production in bean petiole explants in the light. JA-Me had no effect on neutral sugar compositions and the quantities of pectic and hemicellulosic polysaccharides of cell walls in the abscission zone. However, the contents of cellulosic polysaccharides were significantly reduced by JA-Me. In the abscission zone, JA-Me accelerated the decrease of the level of UDP-sugars prior to the formation of the abscission, which are important key substrates of the synthesis of cell wall polysaccharides. The total activities of cellulase in the pulvinus and the petiole were much higher than those in other parts of 10-day-old bean seedlings.Cellulase activities were inhibited by the addition of IAA or cycloheximide. JA significantly increased the total activities of cellulase in the pulvinus and the petiole. These results suggest that JA and JA-Me did not only promote the degradation of cell wall polysaccharides in the abscission zone, but also inhibited the synthesis of it, and promoted abscission.

茉莉酸（JA）和茉莉酸甲酯（JA-Me）浓度超过1 μ M时，在黑暗和光照条件下均能促进菜豆叶柄外植体的脱落。JA和JA-Me没有促进光照下豆叶柄外植体乙烯的产生。JA-Me对细胞壁中性糖组成和果胶、半纤维素多糖含量无影响。在水解区，JA-Me加速了水解区形成前UDP-糖水平的下降，而UDP-糖是细胞壁多糖合成的重要底物。10日龄菜豆幼苗叶枕和叶柄中的纤维素酶总活性明显高于其它部位，IAA和放线菌酮对纤维素酶活性有抑制作用。JA显著提高叶枕和叶柄中纤维素酶的总活性。这些结果表明，JA和JA-Me不仅促进水解区细胞壁多糖的降解，而且抑制其合成，促进水解。

项目成果

Ueda,J.,Miyamoto,K.and Aoki,M.: "Jasmonic acid inhibits the IAA-induced elongation of oat coleoptile segments:A possible mechanism involving the metabolism of cell wall polysaccharides." Plant Cell Physiol.35. 1065-1070 (1994)

Ueda,J.、Miyamoto,K. 和 Aoki,M.：“茉莉酸抑制 IAA 诱导的燕麦胚芽鞘节段的伸长：涉及细胞壁多糖代谢的可能机制。”

Ueda,J.,Miyamoto,K.and Aoki,M.: "Jasmonic acid inhibits the IAA-induced elongation of oat coleoptile segments:A possible mechanism involvingthe metabolism of cell wall" Plant Cell Physiol.35. 1065-1070 (1994)

Ueda, J.、Miyamoto, K. 和 Aoki, M.：“茉莉酸抑制 IAA 诱导的燕麦胚芽鞘节段的伸长：涉及细胞壁代谢的可能机制”Plant Cell Physiol.35。

Junichi Ueda: "Separation of a new type of plant growth regulator,jasmonates,by chromatographic procedures" J.Chromatogr.658. 129-142 (1993)

Junichi Ueda：“通过色谱程序分离新型植物生长调节剂茉莉酸”J.Chromatogr.658。

Ueda, J., Miyamoto, K.and Kamisaka, S.: "Separation of a new type of plant growth regulator, jasmonates, by chromatographic procedures" J.Chromatogr.658. 129-142 (1994)

Ueda, J.、Miyamoto, K. 和 Kamisaka, S.：“通过色谱程序分离新型植物生长调节剂茉莉酸”J.Chromatogr.658。

Ueda, J., Miyamoto, K.and Aoki, M.: "Jasmonic acid inhibits the IAA-induced elongation of oat coleoptile segments : A possible mechanism involving the metabolism of cell wall polysaccharides" Plant Cell Physiol.35. 1065-1070 (1994)

Ueda, J.、Miyamoto, K. 和 Aoki, M.：“茉莉酸抑制 IAA 诱导的燕麦胚芽鞘片段的伸长：涉及细胞壁多糖代谢的可能机制”Plant Cell Physiol.35。