Fluorescence Determination of Optical Purity of Aminoacid Ester using Fluorescence Labeled Chiral Host Molecules

使用荧光标记的手性主体分子荧光测定氨基酸酯的光学纯度

• 批准号: 06640771
• 负责人: JIN Takashi
• 金额: $ 1.28万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640771/
• 关键词: Optical actibity; Fuorescence; Host molecule; Aminoacid; Optical purity; キラル; カリックスアレン; 光学分割; 分子認識; ピレン; センサー

In order to develop a new fluorescence technique for the determenation of optical purity of chiral compounds, chiral calix [4] arenes labeled fluorescent moieties (pyrene) were synthesized as fluorescent indicators which interact with aminoacid ether derivatives. Optical resolution of the fluorescent chiral calix [4] arenes were carriedout by using HPLC with the aid of a chiral column. Optical activity of thesynthesized calixarenes were confirmedby the measurement of circular dechrorism of the compounds. Although the fluorescent chiral calix [4] arenes does not exhibit high sensitivities in the complexation with aminoacid ether derivatives, this research provides a basicidea for the determination of optical purity of chiral compounds using fluorescent host molecules as chiral indicators.

为了发展一种新的手性化合物光学纯度测定的荧光技术，合成了手性杯[4]芳烃标记的荧光基团（芘）作为荧光指示剂，与氨基酸醚衍生物相互作用。本文采用高效液相色谱手性柱对荧光手性杯[4]芳烃进行了光学拆分。合成的杯芳烃化合物的旋光性通过环向色变的测定得到了证实。虽然荧光手性杯[4]芳烃与氨基酸醚衍生物的络合灵敏度不高，但本研究为荧光主体分子作为手性指示剂测定手性化合物的光学纯度提供了一种基本思路。

项目成果

O.Inanami, T.Asanuma, N.Inukai, T.Jin, S.Shimokawa, N.Kasai, M.Nakano, F.Sato, and M.Kuwabara: "The Suppression of Aged-related Accumulation of Lipid Peroxidates in Rat Brain by Administration of Rooibos Tea (Asoalathus linearis)" Neuroscience Letters.196

O.Inanami、T.Asanuma、N.Inukai、T.Jin、S.Shimokawa、N.Kasai、M.Nakano、F.Sato 和 M.Kuwabara：“抑制大鼠中与年龄相关的脂质过氧化物积累

Minoru Inabayashi, Soiji, Miyauch, Naoki, Kamo, and Takashi Jin: "Conductance change in Phospho Iipid Bilager Hembrane by an Electricalneutral Ionophore, Monensin" Biochemistry. 34. 3455-3460 (1995)

Minoru Inabayashi、Soiji、Miyauch、Naoki、Kamo 和 Takashi Jin：“电中性离子载体莫能菌素引起的磷酸脂 Bilager Hembrane 的电导变化”生物化学。

M. Kuwabara, T. Asenuma, O. Inanami, T. Jin. S. Shimokawa, N, Kasai, K. Kator, and F. Sato: "Magnetic Resonance Imaging of Young and Aged Rat Brains under a Magnetic Pield of 7.05T" J. Yet. Med. Sci.55(6). 933-938 (1994)

M.桑原，T. Asenuma，O. Inanami，T. Jin。

M.Inabayashi,S.Miyauchi,N,Kamo,and T.Jin: "Clnductance Change in Phos pholipid Bilayer Membranes by an Electricalneutral Ionophore,Monensin" Biochemistry. 34. 3455-3460 (1995)

M.Inabayashi、S.Miyauchi、N、Kamo 和 T.Jin：“电中性离子载体莫能菌素对磷脂双层膜的电导变化”生物化学。

M.Kuwabara,T.Asanyma,O.Inanami,T.Jin,S.Shimokawa,N.Kasai,K.Kator,and F.Sato: "Magnetic Resonanee Inaging of Young and Aged Rat Brains under a Magnetic Field of P.05 T" J.ret.Mrd.Sci.55(6). 933-938 (1994)

M.Kuwabara、T.Asanyma、O.Inanami、T.Jin、S.Shimokawa、N.Kasai、K.Kator 和 F.Sato：“P 磁场下年轻和老年大鼠大脑的磁共振成像”。