Isolation and Molecular Phyogeny of the Most primitive Eukaryotes in Algae

藻类中最原始真核生物的分离和分子系统发育

• 批准号: 06640910
• 负责人: NAGASHIMA Hideyuki
• 金额: $ 1.22万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640910/
• 关键词: Primitive; Eukaryote; Red algae; Cyanidium; Galdieria; 18_S ribosome RNA; Molecular Phylogeny; 分子系統学; 温泉藻; 熱川温泉; 峰温泉; 18S リボソームRNA; 分子系統樹; 温泉藻類; 那須温泉

Unicellular algae, Cyanidium caldarium and Galdieria sulphuraria were isolated from a acid-hot springs, Nasu, Yumoto spa, Tochigi prefecture in 1994. Blue-green algae (Cyanobacteria), Synechococcus, Phormidium and Oscillatoria were found in hot-springs at neutral pH, such as Atagawa, Mine and Shuzenji spas, Izu peninsula in 1995.Then, total DNA were extracted from C.caldarium, G.sulphuraria and C.merolae and 18s rRNA genes of them were multiplied by using PCR.As a result, the gene was much obtained from C.caldarium. Partial DNA sequence of the gene of C.caldarium are considerably different from those of other red algae. We postulate that C.caldarium may be far from standard red algae phylogenetically.

1994年，从樟木县汤本温泉那须的酸性温泉中分离出单细胞藻类，即高温Cyanidium caldarium和Galdieria sulphuraria。1995年在日本伊豆半岛的热川温泉、矿山温泉、修善寺温泉等中性温泉中发现了蓝绿藻、聚球藻、席藻和颤藻等。高温藻基因的部分DNA序列与其它红藻有较大差异。我们推测，C.caldarium可能远不是标准的红藻。

项目成果

長島秀行: "群馬県草津温泉の微細藻類" 温泉科学. 45. 26-30 (1995)

长岛英幸：“群马县草津温泉的微藻”《温泉科学》45. 26-30 (1995)。

H.Nagashima: "Microalgae in Kusatsu Spa, Gumma Prefecture" J.Balineological Soc. Jpn. Vol.45, No. 1. 26-30

H.Nagashima：“群马县草津温泉的微藻” J.Balineological Soc。

長島 秀行: "群馬県草津温泉の微細藻類" 温泉科学. 45. 26-30 (1995)

长岛英幸：“群马县草津温泉的微藻”《温泉科学》45. 26-30 (1995)。